TRAF4和Eg5在卵巢癌组织中的表达及其临床意义

Expression and Clinical Significance of TRAF4 and Eg5 in Ovarian Cancer

目的探讨TRAF4和Eg5在卵巢癌组织中的表达及其临床意义。方法收集65例行手术治疗的卵巢癌患者术后石蜡包埋组织标本,并选取同期行手术治疗的36例卵巢上皮性良性病变肿瘤组织和36例因子宫肌瘤行全子宫及附件切除术的正常卵巢组织。采用免疫组化法检测组织中TRAF4和Eg5的阳性表达情况,实时荧光定量PCR法检测TRAF4和Eg5 mRNA的表达,Person相关性分析TRAF4和Eg5水平的相关性,通过绘制受试者工作特征曲线(ROC)分析TRAF4和Eg5水平对卵巢癌的诊断效能,Kaplan-Meier曲线分析卵巢癌患者的生存情况。结果卵巢癌组织中TRAF4和Eg5的阳性表达率、TRAF4和Eg5 mRNA明显高于良性病变组织和正常卵巢组织(P<0.001);有淋巴结转移者TRAF4和Eg5 mRNA水平明显高于无淋巴结转移者(P<0.001),FIGO分期中Ⅲ~Ⅳ期TRAF4和Eg5 mRNA水平明显高于Ⅲ~Ⅳ期(P<0.001),浸润程度T3-T4期中TRAF4和Eg5 mRNA水平显著高于T1-T2期(P<0.001);TRAF4和Eg5水平呈正相关性(γ=0.326,P<0.001);TRAF4的ROC曲线面积为0.837(95%CI:0.765~0.908,P<0.001),截断值为1.36时,灵敏度为73.85%,特异度为81.54%;Eg5的ROC曲线面积为0.913(95%CI:0.864~0.961,P<0.001),截断值为1.48时,灵敏度为87.69%,特异度为81.54;TRAF4高表达组5年总生存率为47.06%,明显低于TRAF4低表达组(67.74%)(P<0.05);Eg5高表达组5年总生存率为42.86%,明显低于Eg5低表达组(73.33%)(P<0.05)。结论TRAF4和Eg5在卵巢癌中呈高表达,与淋巴结转移、FIGO分期和浸润程度相关,TRAF4和Eg5的表达检测对卵巢癌的早期诊断和预后具有一定的诊断价值。

Objective To investigate the expression and clinical significance of TRAF4 and Eg5 in ovarian cancer.Methods Paraffin-embedded tissue specimens were collected from 65 patients with ovarian cancer undergoing surgical treatment.36 patients with benign ovarian epithelial lesions undergoing surgical treatment and 36 normal ovarian tissues undergoing total hysterectomy and adrenal resection due to uterine fibroids were selected.Immunohistochemical method was used to detect the positive expression of TRAF4 and Eg5 in tissues.Real time fluorescent quantitative PCR was used to detect the expression of TRAF4 and Eg5 mRNA.Person correlation was used to analyze the correlation between TRAF4 and Eg5 levels.The diagnostic efficacy of TRAF4 and Eg5 levels in ovarian cancer was analyzed by drawing the receiver operating characteristic curve(ROC).Kaplan Meier curve was used to analyze the survival of ovarian cancer patients.Results The levels of TRAF4 and Eg5 mRNA in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(P<0.001);The levels of TRAF4 and Eg5 mRNA in tissues with lymph node metastasis were significantly higher than those in tissues without lymph node metastasis(P<0.001).The levels of TRAF4 and Eg5 mRNA in FIGO stageⅢ~Ⅳwere significantly higher than those in stageⅢ~Ⅳ(P<0.001).The levels of TRAF4 and Eg5 mRNA in T3-T4 stage were significantly higher than those in T1-T2 stage(P<0.001);The levels of TRAF4 and Eg5 were positively correlated(γ=0.326,P<0.001);The ROC curve area of TRAF4 was 0.837(95%CI:0.765-0.908,P<0.001).When the cut-off value was 1.36,the sensitivity was 73.85%,and the specificity was 81.54%;The area of the ROC curve of Eg5 was 0.913(95%CI:0.864-0.961,P<0.001).When the cut-off value was 1.48,the sensitivity was 87.69%,and the specificity was 81.54%;The 5-year overall survival rate of patients with high TRAF4 expression was 47.06%,which was significantly lower than that of patients with low TRAF4 expression 67.74%,(P<0.05);The 5-year overall survival rate of high TRAF4 expression group was 47.06%,significantly lower than that of low TRAF4 expression group(67.74%)(P<0.05).The 5-year overall survival rate of high Eg5 expression group was 42.86%,which was significantly lower than that of low Eg5 expression group(73.33%)(P<0.05).Conclusion TRAF4 and Eg5 are highly expressed in ovarian cancer,which is related to lymph node metastasis,FIGO stage and invasion degree.Detection of TRAF4 and Eg5 expression has certain diagnostic value for early diagnosis and prognosis of ovarian cancer.