结直肠癌组织中HMGB2、MAGE-A9、MMR蛋白表达及其临床意义

Expression and Clinical Significance of HMGB2,MAGE-A9,and MMR Protein inColorectal Cancer Tissues

目的探讨结直肠癌组织中高迁移率族蛋白B2(HMGB2)、黑色素瘤相关抗原-A9(MAGE-A9)、DNA错配修复基因(MMR)蛋白表达情况,以及其在评估患者预后中的临床价值。方法选取52例结直肠癌患者作为研究对象,术中采集所有患者肿瘤标本及癌旁组织标本,检测HMGB2、MAGE-A9、MMR蛋白表达水平,并分析各指标表达情况与病理特征、预后间的关系。结果肿瘤组织内HMGB2、MAGE-A9、MMR阳性表达高于癌旁组织,差异有统计学意义(P<0.05)。肿瘤分期Ⅲ期、分化程度低分化、淋巴结转移患者HMGB2、MAGE-A9、MMR阳性表达率高于Ⅰ期、Ⅱ期、中高分化、无淋巴结转移患者,差异有统计学意义(P<0.05)。随访3年,52例患者存活率为71.15%(37/52);HMGB2阳性组存活率[61.76%(21/34)]低于HMGB2阴性组[88.89%(16/18)],差异有统计学意义(χ^(2)=4.219,P=0.040);MAGE-A9阳性组存活率[58.62%(17/29)]低于MAGE-A9阴性组[86.96%(20/23)],差异有统计学意义(χ^(2)=5.018,P=0.025);MMR阳性组存活率[52.00%(13/25)]低于MMR阴性组[88.89%(24/27)],差异有统计学意义(χ^(2)=8.606,P=0.003)。结论HMGB2、MAGE-A9、MMR在结直肠癌中呈高表达状态,其表达与肿瘤分期、分化程度、淋巴结转移关系密切,且不同表达患者存活率存在较大差异,可作为评估预后的重要指标。

Objective To explore the expression of the high-mobility family protein B2(HMGB2),melanoma-associated antigen-A9(MAGE-A9),and DNA mismatch repair gene(MMR)protein in colorectal cancer tissues,and its clinical value in the evaluation of patient prognosis.Methods 52 CRC patients were selected.Tumor samples and adjacent cancer tissue samples were collected from all patients,and the expression levels of HMGB2,MAGE-A9 and MMR protein were detected,and the relationship between the expression of each index and pathological characteristics and prognosis was analyzed.Results Positive expression of HMGB2,MAGE-A9,and MMR was higher in tumor tissue than in adjacent tissues,The difference was statistically significant(P<0.05);The number of HMGB2,MAGE-A9 and MMR positive cases,which was higher than those with stage I,stage,medium and high differentiation,and no lymph node metastasis,The difference was statistically significant(P<0.05);Follow-up for 3 years,The survival rate of the 52 patients was 71.15%(37/52);The survival rate of the HMGB2-positive group[61.76%(21/34)]was lower than that of the HMGB2-negative group[88.89%(16/18)],The difference was significant(χ^(2)=4.219,P=0.040);The survival rate of the MAGE-A9 positive group[58.62%(17/29)]was lower than that of the MAGE-A9 negative group[86.96%(20/23)],The difference was statistical significant(χ^(2)=5.018,P=0.025);The survival rate of the MMR-positive group[52.00%(13/25)]was lower than that of the MMR-negative group[88.89%(24/27)],The difference was statistically significant(χ^(2)=8.606,P=0.003).Conclusion The expression of HMGB2,MAGE-A9,and MMR in colorectal cancer is closely related to the tumor stage,differentiation degree,and lymph node metastasis,and the survival rate of patients with different expressions varies greatly,which can be used as an important indicator to evaluate the prognosis.