摘要
目的 探讨多发性骨髓瘤(MM)组织中miR-29c、miR-195表达水平变化及其与临床病理特征及预后的关系。方法 选取75例MM患者作为病例组,另选取同期就诊的75例骨髓象检查为正常的非血液系统疾病患者作为对照组。采用实时荧光定量PCR(q-PCR)检测骨髓组织中miR-29c、miR-195表达水平,根据检测平均值将miR-29c、miR-195分为高水平组和低水平组,分析miR-29c、miR-195表达水平与临床病理特征及预后的相关性。结果 病例组骨髓组织miR-29c、miR-195表达水平低于对照组(P<0.05)。miR-29c低水平组中ISS分期Ⅲ期及血清白蛋白水平<35 g/L的患者占比高于高水平组(P<0.05);miR-195低水平组中ISS分期Ⅲ期及血清白蛋白水平<35 g/L的患者占比高于高水平组(P<0.05);miR-29c低水平组的生存期低于高水平组(P<0.05),miR-195低水平组的生存期低于高水平组(P<0.05)。miR-29c、miR-195表达水平与ISS分期呈负相关(P<0.05),与血清白蛋白、生存期呈正相关(P<0.05)。结论miR-29c、miR-195均在MM患者骨髓组织中低表达,且低表达miR-29c、miR-195患者的生存期较短。miR-29c、miR-195表达水平与ISS分期呈正相关,与血清白蛋白、生存期呈负相关。
Objective To investigate the expression of miR-29c and miR-195 in multiple myeloma(MM)and their relationship with clinicopathological features and prognosis.Methods 75 patients with MM were selected as the case group,and 75 patients with non-hematological diseases with normal bone marrow imaging were selected as the control group.Detection of miR-29c and miR-195 expression in bone marrow tissue tissues by real-time fluorescent quantitative PCR(q-PCR).MiR-29c and miR-195 were divided into high level group and low level group according to the average value of detection.Analysis of the correlation between miR-29c,miR-195 expression levels and clinicopathological characteristics and prognosis.Results The expression levels of miR-29c and miR-195 in bone marrow tissues of the case group was higher than that in the control group(P<0.05).The proportion of patients with ISS stageⅢand serum albumin level<35 g/L in the low level group of miR-29c was higher than that in the high level group(P<0.05).The proportion of patients with ISS stageⅢand serum albumin level<35g/L in the low level group of miR-195 was higher than that in the high level group(P<0.05).The survival time of the low level group of miR-29c was lower than that of the high level group(P<0.05),and the survival time of the low level group of miR-195 was lower than that of the high level group(P<0.05).The expression level of miR-29c and miR-195 was negatively correlated with ISSⅢ(P<0.05),and positively correlated with serum albumin and survival(P<0.05).Conclusion The expression of miR-29c and miR-195 is low in bone marrow tissue of MM patients,and the survival period of patients with low expression of miR-29c and miR-195 is short.The expression levels of miR-29c and miR-195 are positively correlated with ISSⅢ,and negatively correlated with serum albumin and survival period.
作者
石瑞平
陈兴华
李真
SHI Ruiping;CHEN Xinghua;LI Zhen(Zhengzhou People's Hospital,Zhengzhou,450000)
出处
《实用癌症杂志》
2024年第3期425-429,共5页
The Practical Journal of Cancer