miR-9、miR-194在鼻咽癌组织中的表达及与其预后的关系

Expression of miR-9 and miR-194 in Nasopharyngeal Carcinoma and Their Relationship with Prognosis

目的 探讨miR-9、miR-194在鼻咽癌组织中的表达水平变化及其预后价值。方法 选取鼻咽癌患者76例,随访3~36个月,根据预后情况分为死亡组(n=21例),存活组(n=55例)。统计比较miR-9、miR-194不同表达水平的鼻咽癌患者的临床资料,以及不同预后的鼻咽癌患者miR-9、miR-194表达水平,采用Logistic回归分析鼻咽癌患者预后相关影响因素。结果 76例鼻咽癌患者miR-9高表达29例,低表达47例,miR-194高表达35例,低表达41例;miR-9、miR-194低表达患者的临床分期为Ⅲ~Ⅳ期、肿瘤分期为T3~T4期、组织分化程度为高中分化、发生淋巴结转移及远处转移的比例高于miR-9、miR-194高表达患者(P<0.05);两组临床分期、肿瘤分期、组织分化程度、淋巴结转移及远处转移情况、miR-9表达水平、miR-194表达水平比较差异显著(P<0.05);临床分期、肿瘤分期、组织分化程度、淋巴结转移及远处转移情况、miR-9表达水平、miR-194表达水平为鼻咽癌患者预后影响因素(P<0.05)。结论 miR-9、miR-194低表达与鼻咽癌患者恶性生物学行为进程及预后有关,该研究提示miR-9、miR-194可能是鼻咽癌预后评估的有效分子靶点,值得临床进一步深入探究。

Objective To investigate the changes of expression levels of miR-9 and miR-194 in nasopharyngeal carcinoma and their prognostic value.Methods 76 patients with nasopharyngeal carcinoma were followed up for 3 to 36 months and divided into death group(n=21 cases)and survival group(n=55 cases)according to the prognosis.The clinical data of nasopharyngeal carcinoma patients with different expression levels of miR-9 and miR-194,as well as the expression levels of miR-9 and miR-194 in nasopharyngeal carcinoma patients with different prognosis were statistically compared.Logistic regression was used to analyze the prognostic factors of nasopharyngeal carcinoma patients.Results In 76 patients with nasopharyngeal carcinoma,29 cases showed high expression of miR-9,47 cases showed low expression,35 cases showed high expression of miR-194 and 41 cases showed low expression.The clinical stages of patients with low expression of miR-9 and miR-194 areⅢ~Ⅳ,and the tumor stages are T3~T4.The proportion of patients with high differentiation,lymph node metastasis and distant metastasis was higher than that of patients with high expression of miR-9 and miR-194(P<0.05).There were significant differences in clinical stage,tumor stage,tissue differentiation,lymph node metastasis and distant metastasis,miR-9 expression level and miR-194 expression level between the 2 groups(P<0.05);Clinical stage,tumor stage,degree of tissue differentiation,lymph node metastasis and distant metastasis,miR-9 expression level and miR-194 expression level were the prognostic factors of NPC patients(P<0.05).Conclusion The low expression of miR-9 and miR-194 is related to the malignant biological behavior process and prognosis of nasopharyngeal carcinoma patients.This study suggests that miR-9 and miR-194 may be effective molecular targets for prognosis evaluation of nasopharyngeal carcinoma,which is worthy of further clinical exploration.