水稻抗褐飞虱基因Bph3和Bph24(t)的聚合育种利用

Pyramided Breeding by Use of Nilaparvatal lugens Resistance Genes Bph3 and Bph24(t)in Rice

在水稻(Oryza sativa)的生产实践中,常常会受到褐飞虱(Nilaparvatal lugens)等多种病虫害的威胁。聚合不同抗性基因,培育抗性品系,是应对各种生物胁迫最有效的策略。传统香稻由于其自身抗性等条件限制,无法大面积推广。本研究结合分子标记辅助选择(marker-assisted selection,MAS)技术,以含有抗褐飞虱基因Bph3或Bph24(t)的供体亲本与含有抗稻瘟病基因(Pi2、Pib或Pimh)、抗白叶枯病基因(Xa23)或香味基因(badh2)的优质三系杂交水稻保持系和恢复系进行多亲本复合杂交。将抗褐飞虱基因聚合到优良水稻品系中,筛选获得41个含有抗褐飞虱基因且与其他目的基因以不同组合方式相聚合的稳定品系。抗性鉴定、香味检测和农艺性状测定表明,各聚合品系的褐飞虱抗性水平较受体亲本均有明显提升,且具有双抗、三抗和/或香味等优良表型。这些新品系为多抗、优质水稻新品种的选育提供新的种质材料。

The rice(Oryza sativa)production is affected by many diseases and pests,such as Nilaparvatal lugens.Cultivating resistant cultivars through polymerizing different resistance genes is the most effective strategies to cope with these biotic stresses.Traditional aromatic rice cannot be popularized within a large area due to the deficiency of resistance.In this study,a series of maintainer line and restorer line of high quality three-line hybrid rice,which containing rice blast resistance gene(Pi2,Pib or Pimh),bacterial blight re-sistance gene(Xa23)or aroma gene(badh2)were hybridized with donor parents containing N.lugens resistance gene Bph3 or Bph24(t).As a result,the N.lugens resistance genes were introduced into elite breeding lines through marker-assisted selection(MAS).A total of 41 stable pyramided lines containing N.lugens resistance genes,and aggregated with other target genes in different combina-tions were screened.The identification of resistance,aroma and agronomic traits showed that each pyramided line not only possesses a high level of N.lugens resistance compared to the recipient parents,but also exhibits satisfactory agronomic traits,including dual-re-sistance,triple-resistance and/or aroma.It can be used as germplasm resources for multi resistance and high-quality breeding of rice.