汉中地区天麻萌发菌遗传多样性和促萌效果分析

Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong

以汉中地区9株天麻萌发菌及天麻花粉种子为试材,采用拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等方法,研究了9株天麻萌发菌的遗传多样性、亲缘关系和促萌效果,以期为天麻萌发菌的种质资源管理与评价等提供参考依据。结果表明:9株萌发菌根据两两拮抗试验的程度不同分为不显著、显著和极显著3类;根据ITS序列的差异分为Mycena sp.和Heydenia sp.2类;根据酯酶同工酶酶谱条带在80%的相似水平上可分为5类;9株萌发菌与天麻种子共生萌发,其中C3菌株菌丝生长速度最高为(0.30±0.01)cm·d^(-1),C2菌株的萌发率最高为64.66%±2.89%,C2和C3菌株的综合表现最佳。

The genetic diversity,genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment,ITS sequence determination,esterase isozyme technology and seed mixing,in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types,insignificant,significant and extremely significant according to the degree of antagonism of each pair;according to the difference of ITS sequence,they were classified into Mycena sp.and Heydenia sp.2;according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%;9 germination strains co-germinate with Gastrodia elata seeds,of which the highest growth rate of C3 strain was(0.30±0.01)cm·d^(-1),and the highest germination rate of C2 strain was 64.66%±2.89%,the comprehensive performance of C2 and C3 strains were the best.