基于转录组信息的百合SWEET基因家族鉴定及表达特性分析

Identification and Expression Analysis of Lilium SWEETFamily Genes Based on Transcriptome Data

以东方百合品种‘西伯利亚’为试材,采用生物信息学方法,研究了百合SWEET基因家族成员编码蛋白的理化性质、二级结构、系统进化、保守结构域、Motif及基因表达模式,对百合SWEET基因家族成员进行鉴定,探究其在鳞茎发育过程中的作用,以期为该类基因生物学功能和百合地下茎生鳞茎发育机制的研究提供参考依据。结果表明:从转录组数据鉴定到了12个SWEET基因,所有家族成员都为稳定的疏水蛋白,且均含有MtN3_slv结构域,可划分为4个亚家族,不同成员在百合茎生鳞茎形成和进一步发育中差异表达。

The oriental Lilium hybrids‘Siberia’was used as experimental material.The physical and chemical properties,secondary structure,phylogeny,conserved domains,Motifs and gene expression patterns of SWEETgene family members in Lilium were studied by bioinformatics,the SWEETgene family members of Lilium were identified and explored their role in steam bulblet development,in order to provide reference for the biological function of these genes and further study in molecular mechanism of bulblet formation.The results showed that 12 SWEET genes were identified from the transcriptome data,and all the proteins of the family members were relatively stable hydrophobic proteins,and all contained the MtN3_slv domain,which could be divided into 4subfamilies.Different gene family members performed different expression level during bulblet formation and development stage.