基于中药超分子与肠道菌相互作用探讨大黄-黄连配伍和合的物质基础

Based on the interaction between supramolecules of traditional Chinese medicine and enterobacteria to explore the material basis of combination of Rhei Radix et Rhizoma-Coptidis Rhizoma

基于中药超分子与肠道菌相互作用过程探讨大黄-黄连配伍和合的物质基础。采用扫描电子显微镜和动态光散射法表征大黄单煎液、黄连单煎液与配伍共煎液的形态学差异,通过建立体外抑菌模型(大肠杆菌E.coli,屎肠球菌E.faecium与枯草杆菌B.subtilis)初步评价大黄黄连配伍对肠道菌的损伤作用;通过超高效液相色谱-串联质谱技术分析单煎与共煎的化学组分变化规律。大黄黄连共煎煮后呈浑浊状,电镜下可见300~400 nm的类球形颗粒,且较单煎液相比其汤剂相态更均一稳定;并直接观察到二者配伍后形成的超分子和肠道菌的相互作用与单煎液相比明显不同,超分子与肠道菌相互作用过程中维持类球形状态,封闭着黄连或大黄中的药效成分,可有效缓和对肠道菌的损伤,为后续大黄-黄连配伍“和合”调控肠道菌群稳态研究提供参考。

Based on the interaction between supramolecule of traditional Chinese medicine and enterobacteria,the material basis of Rhei Radix et Rhizoma and Coptidis Rhizoma was explored.Scanning electron microscopy(SEM)and dynamic light scattering(DLS)were used to characterize the morphological differences of Rhubarb single decoction,Coptis single decoction and Rhubarb and Coptis co-decoction.An in vitro antibacterial model(E.coli,E.faecium and B.subtilis)was established to evaluate the damage effect of the combination of Rhei Radix et Rhizoma and Coptidis Rhizoma on enterobacteria.Ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to analyze the changes of chemical components of single decoctions and co-decoctions.The co-decoction of Rhei Radix et Rhizoma and Coptidis Rhizoma was turbid after decocting.The spherical particles of 300-400 nm were observed under SEM,and the co-decoction was more uniform and stable than that of single decoction.The interaction between supramolecules formed after the combination of Rhei Radix et Rhizoma and Coptidis Rhizoma and enterobacteria was significantly different from that of single decoction.In the process of interaction between supramolecules and enterobacteria,the spherical state was maintained,and the medicinal ingredients in Coptidis Rhizoma or Rhei Radix et Rhizoma were blocked,which could effectively alleviate the damage to enterobacteria.This study provided a reference for subsequent studies on the regulation of intestinal flora homeostasis by the combination of Rhei Radix et Rhizoma and Coptidis Rhizoma.