UPF3B促进上消化道恶性肿瘤细胞增殖

UPF3B promotes proliferation of malignant tumor cells in upper gastrointestinal tract

目的探讨UPF3B对上消化道恶性肿瘤细胞增殖的影响。方法通过qRT-PCR和Western Blot技术验证胃癌和食管癌细胞中UPF3B的敲低和过表达效率;CCK-8、EdU、平板克隆形成实验评估细胞的增殖能力。结果在胃癌和食管癌细胞中通过siRNA敲低UPF3B的表达水平,UPF3B的mRNA和蛋白表达水平显著降低,且有统计学意义(P<0.001),CCK8、EdU、平板克隆形成实验结果表明敲低UPF3B抑制胃癌和食管癌细胞的增殖;在胃癌和食管癌细胞中转染UPF3B过表达质粒上调UPF3B的表达水平,UPF3B的mRNA和蛋白表达水平显著升高,且有统计学意义(P<0.001),CCK8、EdU、平板克隆形成实验结果表明过表达UPF3B促进胃癌和食管癌细胞的增殖。结论UPF3B促进上消化道恶性肿瘤细胞增殖。

Objective To investigate the effect of UPF3B on the proliferation of malignant cells inUpper gastrointestinal malignant tract.Methods Interference and overexpression efficiency of UPF3B in gastric and esophageal cancer cells verified by qRT-PCR and Western blot;the proliferation ability of cells was detected by CCK-8,EdU,and plate colony formation assay.Results siRNA knockdown of UPF3B expression levels in gastric and esophageal cancer cells significantly decreased the mRNA and protein expression levels of UPF3B,and was statistically significant(P<0.001),and CCK8,EdU and plate colony formation assay showed that knockdown of UPF3B inhibited the proliferation of gastric and esophageal cancer cells;transfection of UPF3B overexpression plasmid in gastric and esophageal cancer cells up-regulated the expression levels of UPF3B,and the mRNA and protein expression levels of UPF3B were significantly increased,and was statistically significant(P<0.001),and the results of CCK8,EdU and plate colony formation assay showed that overexpression of UPF3B promoted the proliferation of gastric and esophageal cancer cells.Conclusion UPF3B promotes the proliferation of malignant tumor cells in upper gastrointestinal tract.