LRRC59在宫颈癌中的表达及预后价值

Expression and prognostic value of LRRC59 in cervical cancer

目的:评估富含亮氨酸重复序列蛋白59(LRRC59)在宫颈癌组织中的表达及对宫颈癌患者预后的影响,并探讨LRRC59调节宫颈癌发生的机制。方法:GEPIA网站分析LRRC59 mRNA在13例正常宫颈组织和306例宫颈癌组织中的表达水平。实时荧光定量聚合酶链反应检测LRRC59 mRNA在10例正常宫颈组织及12例宫颈癌组织中的表达差异。Kaplan-Meier Plotter数据库分析LRRC59对宫颈癌患者总生存期和无复发生存期的影响。基于TCGA数据库中的转录组数据和临床数据绘制ROC曲线并进行单因素和多因素Cox回归分析。基因集富集分析探究LRRC59调节宫颈癌发生的机制。结果:LRRC59 mRNA在宫颈癌组织中表达增加(P<0.05)。LRRC59高表达的宫颈癌患者总生存期(P=0.022)及无复发生存期(P<0.001)更短。LRRC59对宫颈癌患者预后有一定的诊断价值(3年AUC=0.610,5年AUC=0.633)。LRRC59高表达是宫颈癌患者预后不良的独立危险因素[HR=2.645,95%CI(1.378~5.078),P=0.003]。LRRC59通过多条肿瘤相关的信号通路调节宫颈癌的发生。结论:LRRC59在宫颈癌组织中表达增加,LRRC59高表达与宫颈癌患者预后不良相关,可能是宫颈癌新的治疗靶点。

Objective:To evaluate the expression level,prognostic value and potential mechanisms of leucine-rich repeat-containing protein 59(LRRC59)in cervical cancer.Methods:GEPIA database was used to examine the expression level of LRRC59 mRNA in 13 normal cervical tissues and 306 cervical cancer tissues.Real-time quantitative polymerase chain reaction was conducted to detect the expression of LRRC59 mRNA in 10 normal cervical tissues and 12 cervical cancer tissues.Kaplan-Meier Plotter database was used to analyze the relationship between LRRC59 expression and overall survival and relapse-free survival of cervical cancer patients.ROC curve was plotted and univariate and multivariate Cox regression analysis were performed using the transcriptome and clinical data from TCGA database.Gene set enrichment analysis was used to explore the mechanisms of LRRC59 in cervical cancer.Results:The expression of LRRC59 mRNA was increased in cervical cancer tissues compared with normal cervical tissues(P<0.05).The patients with high expression of LRRC59 had a shorter overall survival(P=0.022)and relapse-free survival(P<0.001).LRRC59 had a certain diagnostic value in the prognosis of cervical cancer patients(AUC at 3 years was 0.610,AUC at 5 years was 0.633).The high expression of LRRC59 was an independent risk factor predicting poor prognosis in cervical cancer patients[HR=2.645,95%CI(1.378~5.078),P=0.003].LRRC59 regulated the development of cervical cancer through multiple tumor-associated signaling pathways.Conclusion:The expression of LRRC59 mRNA is increased in cervical cancer tissues,and the high expression of LRRC59 is associated with poor prognosis in cervical cancer patients.LRRC59 may be a novel therapeutic target for cervical cancer.