多色比色法在生物传感平台的研究进展

Research Progress of Multicolor Colorimetric Method in Biosensing Platform

比色法是生物传感中较为常用的一种检测手段,因其具有较高的灵敏度和特异性而受到广泛关注。其中,基于多色探针的多色比色法已成为近年来的研究热点,灵敏度较单色比色法显著提高、且具有更高的分辨率和肉眼辨识度,通过观察颜色变化可进行可视化半定量检测,又可通过计算颜色比率或基于智能手机图像处理软件进行快速定量检测。基于有机小分子荧光显色、纳米材料显色及酶促反应显色3种不同的显色机理,综述了多色荧光小分子探针、多色纳米材料比色探针及多色酶促反应比色探针的检测机理以及它们在实际样本检测中的应用,并进一步探讨了其面临的挑战及未来发展机遇。

The colorimetric method is a common detection method in biosensing,which has received much attention because of its high sensitivity and specificity.Among them,multicolor colorimetric method based on multicolor probes has become a hot research topic in recent years.Its sensitivity is significantly higher than that of monochromatic colorimetric method,and it has higher resolution which can be visualized for semi-quantitative detection by naked eye recognition,quickly quantified by calculating color ratios or based on smartphone image processing software.Based on three different color development mechanisms:organic small molecule fluorescence,nanomaterial color development and enzymatic reaction color development,the detection mechanisms of multicolor fluorescent small molecule probes,multicolor nanomaterial colorimetric probes and multicolor enzymatic reaction colorimetric probes are reviewed and their applications in practical sample detection are discussed.The challenges and future development opportunities are also discussed.