骨髓增生异常综合征CD34+细胞焦亡相关分子焦孔素E研究

GSDME,a molecule related to pyroptosis of CD34+cells in patients with myelodysplastic syndrome

目的探讨骨髓增生异常综合征(MDS)患者骨髓CD34+细胞中焦孔素E(GSDME)与胱天蛋白酶-3(Caspase-3)表达水平。方法选取2020年9月至2021年12月在天津医科大学总医院血液科收治的32例初治MDS患者与20例正常者对照,利用流式细胞术、qRT-PCR技术检测骨髓CD34+细胞中GSDME与Caspase-3表达水平。结果流式细胞术结果显示:初治低危组骨髓CD34+细胞中GSDME与活化Caspase-3的表达水平[(64.90±15.74)%与(31.83±17.99)%]显著高于对照组[(41.39±14.07)%与(24.11±16.28)%,P<0.05]。初治高危组GSDME与活化Caspase-3的表达水平[(30.69±14.62)%与(15.42±13.34)%]显著低于对照组与初治低危组(P<0.05)。qRT-PCR结果显示:高危MDS患者骨髓CD34+细胞GSDME基因相对表达量低于对照组(0.20±0.19比1.00±0.53,P<0.05),但Caspase-3基因相对表达量无明显差别。结论低危MDS患者骨髓CD34+细胞内GSDME表达水平升高,细胞焦亡水平可能升高,导致骨髓造血衰竭;高危MDS患者骨髓CD34+细胞内GSDME表达水平降低,细胞焦亡水平可能降低,细胞过度增殖,向白血病转化的风险更大。

Objective To investigate the expression levels of GSDME and Caspase-3 in bone marrow CD34+cells of myelodysplastic syndrome(MDS)patients.Methods From September 2020 to December 2021,20 normal controls and 32 patients with myelodysplastic syndrome admitted to the hematology department of Tianjin Medical University General Hospital were compared,expression of GSDME and Caspase-3 in bone marrow CD34+cells was detected by Flow Cytometry and qRT-PCR.Results According to the Flow Cytometry,the expression levels of GSDME and Cleaved Caspase-3 of CD34+cells in bone marrow of low risk group were increased[(64.90±15.74)%,(31.83±17.99)%]than the control group[(41.39±14.07)%,(24.11±16.28)%,P<0.05];The expression of GSDME and Cleaved Caspase-3 in the high risk group[(30.69±14.62)%,(15.42±13.34)%,P<0.05]were decreased.According to the result of qRT-PCR,the relative expression of GSDME(0.20±0.19 vs.1.00±0.53,P<0.05)gene in high risk MDS patients was significantly decreased,but there was no significant difference in the expression of Caspase-3.Conclusion In low risk MDS patients,the expression of GSDME in CD34+cells increase,the level of pyroptosis may increase,leading to bone marrow hematopoietic failure.In high risk MDS patients,the expression of GSDME in CD34+cells decrease,the level of pyroptosis may decrease,the cells may proliferate excessively,and the risk of leukemia transformation is increased.