白术内酯Ⅰ调节IL-6/STAT3信号通路对子宫内膜癌细胞增殖、迁移和侵袭的影响

Impacts of atractylenolide-I on the proliferation,migration and invasion of endometrial carcinoma cells by regulating the IL-6/STAT3 signaling pathway

目的探讨白术内酯Ⅰ调节白细胞介素-6(IL-6)/信号传导及转录激活蛋白3(STAT3)信号通路对子宫内膜癌细胞增殖、迁移和侵袭的影响。方法用不同浓度白术内酯Ⅰ(0、25、50、75、100、150μmol/L)处理子宫内膜RL-952细胞,MTT法检测细胞存活率;将RL-952细胞分为对照组(无处理)、白术内酯Ⅰ(50μmol/L)组、IL-6组(10 ng/mL)、白术内酯Ⅰ(50μmol/L)+IL-6组(10 ng/mL),MTT法、Edu染色检测细胞增殖;qRT-PCR法检测细胞中IL-6、STAT3表达水平;Transwell实验检测细胞迁移和侵袭;采用流式细胞术检测细胞凋亡率;western blot检测MMP-2、Ki-67、IL-6、p-STAT3、STAT3蛋白表达水平。结果与0μmol/L比较,25μmol/L、50μmol/L、75μmol/L、100μmol/L、150μmol/L白术内酯Ⅰ干预的RL-952细胞存活率显著下降,呈浓度依赖性(P<0.05);与对照组比较,白术内酯Ⅰ组RL-952细胞活力、增殖率、迁移和侵袭个数、IL-6和STAT3 mRNA表达、MMP-2、Ki-67、IL-6、p-STAT3/STAT3蛋白表达水平显著性降低(P<0.05),细胞凋亡率显著性升高(P<0.05),IL-6组RL-952细胞活力、增殖率、迁移和侵袭个数、IL-6和STAT3 mRNA表达、MMP-2、Ki-67、IL-6、p-STAT3/STAT3蛋白表达水平显著性升高(P<0.05),细胞凋亡率显著性降低(P<0.05);与白术内酯Ⅰ组比较,白术内酯Ⅰ+IL-6组RL-952细胞活力、增殖率、迁移和侵袭个数、IL-6和STAT3 mRNA表达、MMP-2、Ki-67、IL-6、p-STAT3/STAT3蛋白表达水平显著性升高(P<0.05),细胞凋亡率显著性降低(P<0.05);与IL-6组比较,白术内酯Ⅰ+IL-6组RL-952细胞活力、增殖率、迁移和侵袭个数、IL-6和STAT3 mRNA表达、MMP-2、Ki-67、IL-6、p-STAT3/STAT3蛋白表达水平显著性降低(P<0.05),细胞凋亡率显著性升高(P<0.05)。结论白术内酯Ⅰ可能通过抑制IL-6/STAT3信号通路来抑制子宫内膜癌RL-952细胞增殖、迁移和侵袭。

Objective To investigate the impacts of atractylenolide-I(AT-I)on the proliferation,migration and invasion of endometrial cancer cells by regulating the interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods The endometrial cancer cell line RL95-2 was induced with AT-I at varying concentrations(0,25,50,75,100 and 150μmol/L),followed by the detection of cell survival rate by MTT assay.Then,RL95-2 cells were induced with blank control,50μmol/L AT-I,10ng/mL IL-6 and 50μmol/L AT-I+10ng/mL IL-6.Cell proliferation was detected by MTT assay and EdU staining.The relative expressions of IL-6 and STAT3 in cells were detected by quantitative reverse transcriptase PCR(qRT-PCR).Transwell assay was applied to detect cell migration and invasion.The apoptotic rate was detected by flow cytometry Western blot was applied to detect the protein levels of matrix metalloproteinase(MMP)-2,Ki-67,IL-6,p-STAT3 and STAT3.Results Compared with cells induced with blank control,the survival rate of RL95-2 cells treated with 25μmol/L,50μmol/L,75μmol/L,100μmol/L,and 150μmol/L AT-I dose-dependently decreased(P<0.05).Compared with those of the control group,RL95-2 cells induced with 50μmol/L AT-I presented significantly lower cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly higher apoptotic rate(all P<0.05).Compared with those of the control group,RL95-2 cells induced with 10ng/mL IL-6 presented significantly higher cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly lower apoptotic rate(all P<0.05).Compared with those induced with 50μmol/L AT-I,RL95-2 cells induced with induced with 50μmol/L AT-I+10ng/mL IL-6 presented significantly higher cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly lower apoptotic rate(all P<0.05).Compared with those induced with 10ng/mL IL-6,RL95-2 cells induced with induced with 50μmol/L AT-I+10ng/mL IL-6 presented significantly lower cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly higher apoptotic rate(all P<0.05).Conclusion AT-I may inhibit the proliferation,migration and invasion of the endometrial cancer cell line RL95-2 by inhibiting the IL-6/STAT3 signaling pathway.