新型可视喉镜在小鼠气管插管中的应用研究

Application of a Novel Visual Laryngoscope in Endotracheal Intubation in Mice

目的:评价新型可视喉镜在制备脂多糖(lipopolysaccharide, LPS)诱导的小鼠急性呼吸窘迫综合征(acute respiratory distress syndrome, ARDS)模型中的应用效果。方法:SPF级健康C57BL/6雄鼠40只,采用随机数字表法将其分为4组(n=10):VP组(新型可视喉镜辅助下气管插管滴注PBS)、VL组(新型可视喉镜辅助下气管插管滴注LPS)、LP组(胸部透光法气管插管滴注PBS)、LL组(胸部透光法气管插管滴注LPS)。记录制备ARDS模型的气管插管时间、气管插管成功率及有无气管插管不良反应。24 h后处死小鼠,收集肺组织行HE染色观察病理学变化,并进行肺损伤评分。收集支气管肺泡灌洗液(BALF),采用BCA法检测总蛋白浓度,采用ELISA法检测TNF-α、IL-6、IL-1β浓度。结果:与LP组和LL组比较,VP组和VL组的气管插管时间缩短,一次成功率提高,差异有统计学意义(P<0.05);与VP组和LP组对比,VL组和LL组的肺损伤评分、BALF总蛋白浓度、中性粒细胞计数及TNF-α、IL-6、IL-1β浓度均显著升高,差异有统计学意义(P<0.05);与LL组对比,VL组在上述检测指标中的结果比较差异无统计学意义(P>0.05)。结论:新型可视喉镜在制备LPS诱导的小鼠ARDS模型中操作更简单快捷、模型成功率更高,并且不影响模型的制备效果,具有推广意义。

Objective To evaluate the effectiveness of a novel visual laryngoscope in the preparation of a lipopolysaccharide(LPS)induced acute respiratory distress syndrome(ARDS)model in mice.Methods Forty healthy SPF-grade male C57BL/6 mice of 8-10 weeks old and weighing 22-25 g were randomized into 4 groups(n=10):visual laryngoscopy-as⁃sisted endotracheal intubation with PBS(VP),visual laryngoscopy-assisted endotracheal intubation with LPS(VL),chest transillumination endotracheal intubation with PBS(LP)and chest transillumination endotracheal intubation with LPS(LL).The duration success rate and the presence of adverse effects of intubation were recorded in the preparation of the ARDS model.The mice were executed 24 h after successful endotracheal intubation,and the lung tissue samples were col⁃lected for HE staining to observe the pathological changes and to score the lung injury.The bronchoalveolar lavage fluid(BALF)was collected for the determination of total protein concentration by the BCA method and TNF-α,IL-6 and IL-1βconcentrations by ELISA.Results Compared with the LP and LL groups,the VP and VL groups had reduced intubation duration and increased primary success rate(P<0.05).The VL and LL groups had higher lung injury scores,BALF total protein,neutrophil count and TNF-α,IL-6 and IL-1βconcentrations than the VP and LP groups(P<0.05).There were no significant differences in the above indicators between the LL and VL groups(P>0.05).Conclusion The novel visual la⁃ryngoscope with simpler operation,higher modeling success rate and uncompromised modeling performance in the prepara⁃tion of LPS-induced ARDS model in mice is of great significance for popularization.