玉米开花促进基因ZmGI及其同源蛋白结构特征分析和功能预测

Structural Characterization Analysis and Functional Prediction of the Maize Flowering Factor ZmGI and Relevant Cognate Proteins

光周期是调控植物开花的重要因子之一,对植物的生长发育具有重要影响。为更好地揭示开花促进基因Zm GI在玉米中的功能,本研究以12个GI蛋白作为靶标,利用生物信息学分析方法,对GI各编码蛋白的理化性质、保守基序、蛋白质二级结构、基因结构、上游启动子顺式调控元件等信息进行分析,旨在揭示Zm GI的表达模式。结果表明,同一亚群的基因结构相似度更高。12个同源蛋白表现出:(1)具有10个不同的保守模体;(2)不具有跨膜结构域的特征。亚细胞定位结果预测表明,GI蛋白可能定位于细胞核和叶绿体中。互作蛋白分析表明,具有CCT结构域的基因可能与Zm GI共表达于细胞中。组织表达模式分析发现,Zm GI在成熟叶片中显著表达。启动子分析结果显示,GI上游启动子中存在大量的逆境响应元件和光响应元件。这些结果将为深度解析Zm GI基因的功能提供更丰富的理论支撑。

Photoperiod is one of the most important factors regulating plant flowering,which has an important influence on plant growth and development.In order to clarified the function of ZmGI and relevant expression model in maize,in this study,12 GI proteins were selected to be targets,and relevant physical and chemical properties,conservative mods,protein secondary structure,gene structure,upstream promoter cis-regulatory elements and so on of proteins encoded by GI gene were identifed by using bioinformatics analysis method.The results showed that the similar structure characterization were found among genes apionted into the same subpopulation.For the 12 homologous proteins:(1)There are 10 different conserved motifs;(2)It does not have the characteristics of transmembrane domain.Subcellular localization results showed that GI protein might be located in nucleus and chloroplast.Interaction protein analysis showed that the genes with CCT domain might be co-expressed with ZmGI.Tissue expression pattern analysis showed that ZmGI was significantly expressed in mature leaves.Promoter analysis showed that there were a large number of stress-responsive and light-responsive elements in the upstream promoter region of GI.These results would provide much more theoretical supports for resolving function of ZmGI gene deeply.