植物源性丹酚酸B对递增负荷训练大鼠骨骼肌铁死亡通路的影响

Impact of Plant-Derived Salvianolic Acid B on Ferroptosis Pathway in Rats Skeletal Muscle Induced by Incremental Load Training

研究旨在探究植物源性丹酚酸B对递增负荷训练大鼠骨骼肌铁死亡通路的影响及其潜在的调控机制。本研究从草本植物丹参中提取分离得到纯化的丹酚酸B,按照实验设计喂食给大鼠,随后对大鼠进行递增负荷训练模拟了运动性疲劳状态,通过分析血清标志物和组织生化指标,研究了丹酚酸B在运动性疲劳过程中对氧化应激和铁代谢的影响。研究发现,丹酚酸B可剂量依赖性地减轻递增负荷训练引起的大鼠骨骼肌损伤,其中血清LDH和CK水平分别降低了50%和45%。丹酚酸B显著降低了骨骼肌中MDA水平,减轻了氧化应激。在铁代谢方面,丹酚酸B升高了骨骼肌中GSH水平,并上调了GPX4的转录和蛋白表达水平,抑制了铁死亡。丹酚酸B对铁代谢相关基因的转录和蛋白表达产生明显的影响,降低了Tf、TfR、DMT1的表达水平,提高了FTH1和FPN1的表达水平。结果显示,丹酚酸B通过调控铁代谢通路,参与抗运动性疲劳过程,为丹酚酸B作为天然抗氧化剂和铁死亡抑制剂的潜在机制提供了新见解。

This study aimed to investigate the effects of plant-derived salvianic acid B on the iron death pathway in the skeletal muscles of rats subjected to incremental load training and explore its potential regulatory mechanisms.In this study,salvianic acid B was purified from the herbal plant Danshen,and it was administered to rats following an experimental design.The rats were then subjected to incremental load training to simulate the state of exercise-induced fatigue.The study analyzed serum markers and tissue biochemical indicators to examine the impact of salvianic acid B on oxidative stress and iron metabolism during exercise-induced fatigue.The results revealed that salvianic acid B dose-dependently alleviated skeletal muscle damage induced by incremental load training,reducing serum LDH and CK levels by 50%and 45%,respectively.Salvianic acid B significantly decreased MDA levels in skeletal muscles,mitigating oxidative stress.In terms of iron metabolism,salvianic acid B elevated GSH levels in skeletal muscles and upregulated the transcription and protein expression of GPX4,inhibiting iron death.Salvianic acid B had a noticeable impact on the transcription and protein expression of iron metabolism-related genes,lowering the expression levels of Tf,TfR,and DMT1,while increasing the expression levels of FTH1 and FPN1.The results indicate that salvianic acid B participates in the process of combating exer-cise-induced fatigue by modulating the iron metabolism pathway,providing new insights into the potential mechanisms of salvianic acid B as a natural antioxidant and iron death inhibitor.