新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价

Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth

目的:研究不同浓度新型生物活性玻璃(bioactive glass,BG)对乳牙根尖周炎常见细菌的抗菌效果。方法 :采用纸片扩散法检测并观察新型BG处理粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌后形成的抑菌环直径(mm),同时测定粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌的最小抑菌浓度(minimal inhibitory concentration,MIC)、最小杀菌浓度(minimal bactericidal concentration,MBC)和最小菌斑清除浓度(minimal biofilm eradication concentration,MBEC),20、40、60、80 mg/mL新型BG处理3种菌的混合菌斑24 h,于激光共聚焦显微镜(confocal laser scanning microscopy,CLSM)下摄片观察新型BG对混合菌斑的抑菌效果。采用GraphPad Prism 10.0软件对数据进行统计学分析。结果:新型BG对乳牙根尖周炎常见细菌显示出很强的抗菌潜力;新型BG对菌斑的MBEC显著大于对粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌的MIC和MBC。随着新型BG浓度加大,混合菌斑死菌数量显著增加,与空白对照组有显著差异(P<0.05)。结论:新型BG对乳牙根尖周炎常见细菌粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌具有显著抗菌功效。

PURPOSE:To study the antimicrobial effect of different concentrations of new bioactive glass(BG)on common bacteria in apical periodontitis of deciduous teeth.METHODS:The diameter(mm)of the inhibitory rings formed after treatment of Enterococcus faecalis,Porphyromonas gingivalis and Clostridium nucleatum with the new bioactive glass was detected and observed by paper diffusion method,and the minimal inhibitory concentration(MIC),minimal bactericidal concentration(MBC)and minimal biofilm eradication concentration(MBEC)of E.faecalis,P.gingivalis and C.pseudomallei were determined.The mixed plaques of the three bacteria were treated with 20,40,60 and 80 mg/mL of the new bioactive glass for 24 h.The results were analyzed by laser confocal microscopy.The antibacterial effect of the new bioactive glass on the mixed plaque was observed by confocal laser scanning microscopy(CLSM).Statistical analysis was performed with GraphPad Prism 10.0 software.RESULTS:The new bioactive glass showed strong antibacterial potential against the common bacteria of apical periodontitis;the MBEC of the new bioactive glass on the plaque was significantly greater than MIC and MBC of Enterococcus faecalis,Porphyromonas gingivalis and Clostridium nucleatum,and as the concentration of the new bioactive glass increased,the number of dead bacteria in the mixed plaque increased,and there was significant differ-ence from that of the blank control group(P<0.05).CONCLUSIONS:The novel bioactive glass shows significant antibacterial efficacy against Enterococcus faecalis,Porphyromonas gingivalis and Clostridium nucleatum,which are the common bacteria in apical periodontitis of deciduous teeth.