血清LTBP2、TM4SF1在大肠癌患者预后评估中的研究

Value of Serum Latent Transforming Growth Factor Binding Protein 2 and Transmembrane 4 Superfamily 1 in Prognosis Assessment of Patients with Colorectal Cancer

目的探讨血清转化生长因子结合蛋白2(LTBP2)、四次跨膜蛋白1(TM4SF1)在大肠癌患者预后评估中的意义。方法将南阳市中心医院2016年7月至2019年7月手术治疗的大肠癌患者108例作为试验组,同期108例健康体检者作为对照组。采用酶联免疫吸附试验(ELISA)检测两组血清LTBP2、TM4SF1水平;比较不同血清LTBP2和TM4SF1表达水平的大肠癌患者临床资料的差异;采用免疫组化染色法分析大肠癌组织中LTBP2、TM4SF1的表达;Pearson相关分析明确血清LTBP2和TM4SF1表达的相关性;受试者工作特征(ROC)曲线分析血清LTBP2和TM4SF1联合评估大肠癌患者预后的价值。结果试验组血清LTBP2和TM4SF1水平均高于对照组(P<0.05),二者表达水平呈正相关(r=0.305,P<0.05),大肠癌组织LTBP2和TM4SF1表达阳性率高于癌旁组织(P<0.05)。肿瘤直径>5cm、肿瘤低分化、有淋巴结转移、TNM分期Ⅲ~Ⅳ期、有脉管瘤栓的大肠癌患者血清LTBP2和TM4SF1表达水平高于患者肿瘤直径≤5 cm、肿瘤中高分化、无淋巴结转移、TNM分期Ⅰ~Ⅱ期、无脉管瘤栓的大肠癌患者(P<0.05)。血清LTBP2、TM4SF1联合预测大肠癌患者预后不良的AUC为0.886。结论大肠癌患者血清LTBP2和TM4SF1水平升高,二者联合对大肠癌患者预后不良具有较好的预测价值。

Objective To study the serum levels of latent transforming growth factor binding protein 2(LTBP2)and transmembrane 4 superfamily 1(TM4SF1)and their value in the prognosis assessment of patients with colorectal cancer.Methods A prospective study was conducted.A total of 108 patients with colorectal cancer treated in Nanyang Central Hospital from July 2016 to July 2019 were included in the experimental group,and 108 healthy subjects who underwent physical examination in the same period were included in the control group.The serum levels of LTBP2 and TM4SF1 in both groups were detected by enzyme-linked immunosorbent assay(ELISA).The clinical data of colorectal cancer patients with different expression levels of serum LTBP2 and TM4SF1 were compared.The expression of LTBP2 and TM4SF1 in colorectal cancer tissues was analyzed by immunohistochemical staining.Pearson correlation analysis was applied to analyze the correlation between serum expressions of LTBP2 and TM4SF1.Receiver operating characteristic(ROC)curve was drawn to analyze the evaluation value of the combination of serum LTBP2 and TM4SF1 for poor prognosis in patients with colorectal cancer.Results The serum levels of LTBP2 and TM4SF1 in the experimen-tal group were higher than those in the control group(P<0.05).There was a positive correlation between serum levels of LTBP2 and TM4SF1 in patients with colorectal cancer(r=0.305,P<0.05).The positive expression rates of LTBP2 and TM4SF1 in colorectal cancer tissues were higher than those in adjacent tissues(P<0.05).The expression levels of serum LTBP2 and TM4SF1 in colorectal cancer patients with tumor diameter>5 cm,poorly differentiated tumors,lymph node metastasis,TNM stagesⅢ-Ⅳ,and vascular tumor thrombus were higher than those in colorectal cancer patients with tumor diameter≤5 cm,moderately or highly differentiated tumors,no lymph node metastasis,TNM stagesⅠ-Ⅱ,and no vascular tumor thrombus(P<0.05).The area under the curve(AUC)value of the combination of serum LTBP2 and TM4SF1 for predicting poor prognosis in colorectal cancer patients was 0.886.Conclusion The serum levels of LTBP2 and TM4SF1 are increased in patients with colorectal cancer.And the combination of the two has a good predictive value for the poor prognosis of patients with colorectal cancer.