蓝莓原浆抗氧化及缓解视疲劳功能研究

Studies on Antioxidation and Alleviating Visual Fatigue of Blueberry Pulp

研究蓝莓原浆体内抗氧化及缓解人体视疲劳的功效,分析蓝莓原浆抗氧化功能与改善视疲劳作用的相关性。灌胃给予小鼠低、中、高剂量的蓝莓原浆30 d后,采用试剂盒测定小鼠体内蛋白含量、超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力及丙二醛(MDA)含量。选择18~22岁具有视疲劳症状的志愿者共40名,随机分为对照组与试饮组。试饮组每天饮用2瓶蓝莓原浆,即服用量为100 mL/d,连续服用60 d。试验结果显示:与空白对照组相比,摄入0.53 mL蓝莓原浆后,小鼠体内血清SOD活力显著增加至610.76 U/mg prot,同时小鼠体内血清MDA含量显著降低至4.82 nmol/mg prot(P<0.05);摄入0.21 mL蓝莓原浆可显著增加小鼠体内血清CAT酶活力至8.58 U/mg prot(P<0.05)。人体试饮试验表明饮用本蓝莓原浆60 d后,试饮组视疲劳症状及明视持久度均得到良好改善,其中男性群体具有出显著性(P<0.05)。结论:蓝莓原浆具备较好的抗氧化性及缓解人体视疲劳作用。

To study the antioxidant effect in vivo and the effect of alleviating visual fatigue of blueberry pulp,and analyze the correlation between the antioxidant function of blueberry pulp and the effect of improving visual fatigue.The protein content,superoxide dismutase(SOD)activity,catalase(CAT)activity and malondialdehyde(MDA)content of mice were determined by kit after 30 days of low,medium and high doses of blueberry pulp.A total of 40 volunteers aged 18-22 with visual fatigue symptoms were selected and randomly divided into control group and trial drinking group.The trial drinking group drank 2 bottles of blueberry pulp(100 mL/d)every day for consecutive 60 d.The results showed as follows:Compared with blank control group,the serum SOD activity of mice was significantly increased to 610.76 U/mg prot,while the serum MDA content was significantly decreased to 4.82 nmol/mg prot in the intake of 0.53 mL blueberry pulp(P<0.05),and the intake of 0.21 mL blueberry pulp could significantly increase CAT activity in mice to 8.58 U/mg prot(P<0.05).The human body test showed that after drinking the blueberry juice for 60 d,the symptoms of visual fatigue and the duration of photopic vision were improved in the test group,and there was a significant difference in the male group(P<0.05).Result:Blueberry pulp has good antioxidant properties as well as good for relieving visual fatigue.