氮掺杂碳量子点荧光法检测猪肉中的四环素

Fluorescent Determination of Tetracycline in Pork Based on Nitrogen-doped Carbon Quantum Dots

四环素(TC)作为一种广谱抗生素,用于预防和治疗细菌性疾病,以及作为饲料添加剂促进动物生长。本文旨在建立一种菠萝皮来源的氮掺杂碳量子点(N-CQDs)荧光猝灭法快速检测TC。以菠萝皮和二乙烯三胺为原料,通过水热法制备高量子产率的蓝色荧光N-CQDs。通过透射电子显微镜、傅里叶红外光谱、X射线光电子能谱对N-CQDs进行结构表征。结果表明,N-CQDs的平均粒径为3.53 nm,主要由C、N、O 3种元素组成,表面具有含氧和含氮基团。主要猝灭机理为静态猝灭和内滤效应。在最优试验条件下,该方法在0.3~40μg/mL范围线性良好,检出限为89.26μg/L。该检测方法被用于猪肉中四环素的检测,回收率在98.40%~106.02%之间,相对标准偏差1.29%~3.88%。

As a common antibiotic,tetracycline(TC)is extensively used in the prevention and treatment bacterial diseases in animals and is also used as a feed additive to promote the growth of animals.In this paper,a fluorescence quenching method based on nitrogen doped carbon quantum dots(N-CQDs)derived from pineapple peel was established for the rapid detection of TC.The blue fluorescent N-CQDs with high quantum yield were prepared by the hydrothermal method using pineapple peel and diethylenetriamine as precursors.The structure of N-CQDs was characterized by transmission electron microscope,fourier infrared spectroscopy and X-ray photoelectron spectroscopy.The results showed that the average particle size of N-CQDs was about 3.53 nm,which was mainly composed of C,N and O,and the surface has oxygen-containing and nitrogen-containing groups.The fluorescence of N-CQDs was effectively quenched by static quenching and internal filtering effect.Under the optimal experimental conditions,the method had good linearity within 0.3-40μg/mL,and the detection limit was calculated to be 89.26μg/L.Moreover,the detection method has been applied to the determination of TC in pork,the recovery rate was 98.40%-106.02%.The results showed that the detection method could be applied to the rapid determination of TC in pork.