恒净酸的抑菌效果及对小鼠肠道菌群的影响

Antibacterial Efficacy of Hengjingsuan and Its Impact on Intestinal Flora in Mice

为探讨以枸橼酸、苹果酸、氨基磺酸、甲酸等为主要成分的恒净酸杀菌效果和对小鼠肠道微生物菌群的影响,采用悬液定量杀菌试验等方法观察恒净酸对大肠杆菌、白色念珠菌、金黄色葡萄球菌、铜绿假单胞菌和非洲猪瘟病毒的杀菌效果;采用14 d最大给药量法观察恒净酸的急性毒性反应;通过观察不同稀释度恒净酸对HEK293T细胞形态的影响确定其对细胞的安全浓度;采用高通量测序技术分析恒净酸对小鼠肠道细菌群落结构多样性的影响。结果表明,0.1 g·L^(-1)恒净酸溶液2 min内可有效杀灭大肠杆菌、白色念珠菌、金黄色葡萄球菌、铜绿假单胞菌;1.56 g·L^(-1)恒净酸溶液5 min后可有效灭活非洲猪瘟病毒。恒净酸为实际无毒级物质,对细胞的安全浓度为≤1.56 g·L^(-1)。与对照组相比,恒净酸处理显著提高了拟杆菌门的相对丰度,提高了拟杆菌门/厚壁菌门的比例。综上,恒净酸是一种可有效杀灭病原微生物的新型消毒剂,能改善小鼠的肠道菌群组成,且安全无毒。

To observe the bactericidal efficacy of Hengjingsuan mainly composed of citric acid,malic acid,sulfamic acid and formic acid and its impact on the intestinal microbiota in mice.Suspension quantitative bactericidal test was used to evaluated the bactericidal efficacy of Hengjingsuan on Escherichia coli 8099,Candida albicans ATCC 10231,Staphylococcus aureus CMCC(B)26003,Pseudomonas aeruginosa ATCC 15442 and African Swine Fever Virus(ASFV).The acute toxicity test of Hengjingsuan in mice were was carried out by 14 days maximum dose method and the safe concentration of Hengjingsuan was determined by observing the effect of disinfectant on cell morphology of HEK293T cells and calculating the cell activity.The effect on community structure diversity of intestinal bacteria was analyzed by using high-throughput sequencing technology.The results showed that Hengjingsuan solution at the concentration of 0.1 g·L^(-1) could kill E.coli 8099,C.Albicans ATCC 10231,S.aureus CMCC(B)26003,P.aeruginosa ATCC 15442 effectively within 2 minutes,and it could kill ASFV effectively at 1.56 g·L^(-1) within 5 minutes.Hengjingsuan was an actual non-toxic substance,and its safe concentration for the cell was≤1.56 g·L^(-1).Compared with normal group,the abundance of bacteroidetes significantly increased in mice treated with Hengjingsuan,meanwhile the abundance ratio of bacteroidetes to Firmicutes also increased.Therefore,Hengjingsuan was a new type of disinfectant,which was safe,non-irritating and had great bactericidal efficacy on target pathogenic microorgan.