三个玫瑰品种花色物质的代谢组学分析

Metabolomics analysis of flower color substances in three Rosa rugosa cultivars

玫瑰(Rosa rugosa)具有很高的观赏价值和商业价值,但其花色比较单一,限制了玫瑰的开发利用及其在园林造景中的应用。为了探究‘苦水玫瑰’‘墨红玫瑰’和‘保加利亚白玫瑰’3个不同品种玫瑰的呈色物质,该研究利用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)联用检测花瓣类黄酮的种类和含量,通过KEGG数据库对差异代谢物进行富集分析,筛选出关键代谢物,并分析与花色表型值的相关性。结果表明:(1)在3个不同色系玫瑰花瓣中共检测到58种代谢物,其中花青素只有一种为矢车菊-3-O葡萄糖苷,约占30.45%。(2)K-means聚类分析表明,共有12种关键代谢物注释到KEGG代谢通路中,其中乔松素和杨梅黄酮是决定‘苦水玫瑰’和‘墨红玫瑰’花色呈红色的主要物质,圣草酚、木犀草素和山萘酚是决定‘保加利亚白玫瑰’花色呈白色的主要物质。该研究结果可为具有特定颜色玫瑰的育种提供理论依据,并促进玫瑰在园林绿化中的应用。

Rosa rugosa is a deciduous shrub belonging to Rosa L.in Rosaceae.It has a high ornamental value and commercial value,but its single color limits the development and utilization of rose and its application in landscape architecture.In order to explore the coloring substances of three different varieties of roses,‘Rosa rugosa×Rosa sertata’,‘Rosa Crimson Glory’and‘Rosa alba’,this study used ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)to detect the types and contents of flavonoids in petals.The KEGG database was used to enrich the differential metabolites,screen out the key metabolites,and analyze the correlation with the phenotypic value of flower color.The results were as follows:(1)A total of 58 metabolites were detected in the petals of three different color rose varieties,of which only one anthocyanin was cyanidin-3-O-glucoside,accounting for 30.45%.(2)K-means clustering analysis showed that a total of 12 key metabolites were annotated to the KEGG metabolic pathway.Among them,pinocembrin and myricetin were the main substances that determined the red color of‘Rosa rugosa×Rosa sertata’and‘Rosa Crimson Glory’,and eriodictyol,luteolin and kaempferol were the main substances that determined the white color of‘Rosa alba’.In conclusion,this study can provide a theoretical basis for the breeding of roses with specific colors and promote the application of roses in landscaping.