再生障碍性贫血患者外周血miR-155-5p、miR-1260b水平及临床意义

Levels of miR-155-5p and miR-1260b in peripheral blood of patients with aplastic anemia and their clinical significance

目的探讨再生障碍性贫血(AA)患者外周血微小核糖核酸miR-155-5p、miR-1260b水平及临床意义。方法将该院2014年1月至2019年8月收治的初诊AA患者108例纳入研究作为观察组。另外,选取同期于该院体检的健康志愿者102例作为健康组。采用实时荧光定量聚合酶链反应(qPCR)检测外周血miR-155-5p、miR-1260b水平。比较2组外周血miR-155-5p、miR-1260b水平,观察组中的重型AA与非重型AA患者二者的表达水平。采用Cox回归分析AA患者死亡的影响因素。结果观察组外周血miR-155-5p、miR-1260b水平均高于健康组(P<0.05),重型AA患者二者水平均高于非重型AA患者(P<0.05)。临床分型为重型AA,并发感染、出血,正细胞正色素性贫血、白细胞计数、网织红细胞均值、淋巴细胞与非造血细胞比值、基线血小板计数,外周血miR-155-5p、miR-1260b水平,治疗无效均是AA患者死亡的危险因素(P<0.05)。结论AA患者外周血miR-155-5p、miR-1260b水平升高,与疾病严重程度、预后均相关。

Objective To investigate the levels and clinical significance of microRNA miR-155-5p and miR-1260b in peripheral blood of patients with aplastic anemia(AA).Methods A total of 108 newly diagnosed AA patients admitted to the hospital from January 2014 to August 2019 were enrolled in the study as the observation group.In addition,102 healthy volunteers who underwent physical examination in the hospital during the same period were enrolled as the healthy group.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was used to detect the levels of miR-155-5p and miR-1260b in peripheral blood.The levels of miR-155-5p and miR-1260b in peripheral blood of the two groups were compared,and the expression levels of both in severe AA and non-severe AA patients in the observation group were observed.Cox regression was used to analyze the influencing factors of death in AA patients.Results The levels of miR-155-5p and miR-1260b in peripheral blood of the observation group were higher than those of the healthy group(P<0.05).The levels of miR-155-5p and miR-1260b in severe AA patients were higher than those in non-severe AA patients(P<0.05).The clinical classification of severe AA,complicated with infection,bleeding,normocytic normochromic anemia,white blood cell count,reticulocyte mean value,lymphocyte to non-hematopoietic cell ratio,baseline platelet count,peripheral blood miR-155-5p and miR-1260b levels,and ineffective treatment were all risk factors for the death of AA patients(P<0.05).Conclusion The levels of miR-155-5p and miR-1260b in peripheral blood of AA patients increase,which are related to the severity and prognosis of the disease..