小檗碱经由JAK2/STAT3通路抑制炎症修复脊髓损伤

Berberine Inhibits Inflammation via the JAK2/STAT3 Pathway to Repair Spinal Cord Injury

目的观察小檗碱对脊髓损伤小鼠神经功能恢复的影响并探索其作用机制。方法制备脊髓损伤小鼠模型,采用BMS评分法检测小鼠后肢运动功能;应用NeuN免疫荧光染色观察脊髓组织神经元数量;通过ELISA方法检测IL-6、TNF-α、IL-1β和IL-10蛋白含量;应用qRT-PCR技术检测TNF-α、IL-6、TGF-β1和Arg-1 mRNA的表达变化;采用免疫印迹方法检测P-JAK2、JAK2和P-STAT3、STAT3蛋白含量。结果小檗碱改善脊髓损伤小鼠运动功能,增加神经元数量,减少促炎因子TNF-α、IL-1β和IL-6的分泌,增加抗炎因子IL-10的分泌。小檗碱减少TNF-αmRNA和IL-6 mRNA的表达,增加Arg-1 mRNA和TGF-β1 mRNA的表达。结论小檗碱通过JAK2/STAT3信号通路减轻炎症反应、增加神经元存活,促进脊髓损伤小鼠运动功能恢复。

Objective To observe the effect of berberine on the recovery of nerve function in mice with spinal cord injury and explore its mechanism.Methods The mouse model of spinal cord injury was prepared,and the hind limb motor function was detected by BMS score;the number of neurons in spinal cord was observed by NeuN immunofluorescence staining;the contents of IL-6,TNF-α,IL-1βand IL-10 were detected by ELISA;the mRNA expression of TNF-α,IL-6,TGF-β1 and Arg-1 was detected by qRT-PCR;P-JAK2,JAK2,P-STAT3 and STAT3 proteins were detected by Western Blot.Results Berberine improves motor function in mice with spinal cord injury,increases the number of neurons,decreases the secretion of pro-inflammatory factors TNF-α,IL-1βand IL-6,and increases the secretion of anti-inflammatory factor IL-10.Berberine decreases the expression of TNF-αmRNA and IL-6 mRNA,and increases the expression of Arg-1 mRNA and TGF-β1 mRNA.Conclusion berberine reduces inflammation,increases neuronal survival,and promotes motor function recovery in mice with spinal cord injury through JAK2/STAT3 signaling pathway.