仿生类器官芯片——多学科交叉研究肺纤维化疾病

Biomimetic organoid chips—Interdisciplinary research on pulmonary fibrosis disease

肺纤维化是肺间质单元内肺泡上皮细胞、肺间质固有成纤维细胞、巨噬细胞、炎性细胞、血管内皮细胞与周细胞等多种纤维化行为共同驱动了肺纤维化进展。受限于目前传统的研究技术,体外试验与动物模型均不能对肺脏各型细胞的生物学行为进行实时观察与监测;传统的体外细胞培养模式多与人体肺间质单元的微环境相差甚远,也无法满足研究需求。仿生类器官芯片技术的迅速发展使体外模拟“肺间质”及充分研究“肺纤维化”成为可能。所以本文重点综述类器官芯片的发展及在肺脏模型中的变革与应用,为充分研究肺纤维化疾病提供全新技术平台与检测思路。

Pulmonary fibrosis is driven by various fibrotic behaviors such as alveolar epithelial cells,interstitial fibroblasts,macrophages,inflammatory cells,vascular endothelial cells and pericytes in the interstitial unit.Limited by current traditional research techniques,animal models cannot observe and monitor the biological behavior of lung cells in real time.The traditional in vitro cell culture model is far away from the microenvironment of human lung interstitial unit,and does not meet the research needs.The rapid development of biomimetic organoid chip technology makes it possible to simulate"lung interstitial"in vitro and fully study"pulmonary fibrosis".This paper focuses on the development of organoid chips as well as their changes and applications in lung models.Therefore,it would provide a new technical platform and detection ideas for the full study of pulmonary fibrosis.