太子参-牡丹皮药对治疗糖尿病心肌病网络药理学机制探讨

Study on the Mechanism of Action of Pseudostellariae Radix Pseudostellariae-Moutan bark Medicine in the Treatment of Diabetic Cardiomyopathy based on Network Pharmacology

目的:探究太子参-牡丹皮药对治疗糖尿病心肌病的作用机制。方法:利用网络药理学方法,借助中药系统药理学分析平台(TCMSP)、Pubchem网站、Swiss Target Prediction数据库预测活性成分候选靶点。通过Disgenet和GeneCards数据库筛选出糖尿病心肌病的靶点基因,利用Venny图取中药与疾病的交集靶点,通过STRING数据库建立蛋白互作网络图,并通过Cytoscape 3.9.0软件对网络图进行优化,筛选出核心靶点。利用Metascape数据库对交集靶点进行GO分析和KEGG富集分析。结果:中药共筛选出19个活性成分,277个作用靶点,糖尿病心肌病筛选出疾病靶点2424个,通过韦恩图得出交集靶点109个。通过药物-活性成分-靶点网络图分析可得山奈酚、槲皮素、刺槐素、木犀草素对疾病的治疗可能发挥重要作用。通过PPI网络图可知核心靶点为TNF、AKT1、SRC、TP53、EGFR等。根据KEGG分析,预测该药对主要通过干预癌症、MAPK、PI3K-Akt等信号通路发挥对糖尿病心肌病的治疗作用。结论:太子参-牡丹皮药对可通过多种活性成分、多靶点、多途径对糖尿病心肌病起到保护作用,为糖尿病心肌病的未来防治提供新思路。

Objective:To explore the mechanism of action of Radix Pseudostellariae-Moutan bark medicine in the treatment of diabetic cardiomyopathy.Methods:The network pharmacology method was used to predict active ingredient candidate targets with the help of Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP),Pubchem website,and Swiss Target Prediction database.The target genes of diabetic cardiomyopathy were screened out through the Disgenet and GeneCards databases,the intersection targets of traditional Chinese medicine and diseases were obtained using Venny diagram,the protein interaction network diagram was established through the STRING database,and the network diagram was optimized through Cytoscape 3.9.0 software.Screen out core targets.The Metascape database was used to perform GO analysis and KEGG enrichment analysis on the intersection targets.Results:A total of 19 active ingredients and 277 action targets were screened out for traditional Chinese medicine,2424 disease targets were screened out for diabetic cardiomyopathy,and 109 intersection targets were obtained through the Venn diagram.Through drug-active ingredient-target network diagram analysis,it can be concluded that kaempferol,quercetin,robinin,and luteolin may play an important role in the treatment of diseases.From the PPI network diagram,it can be seen that the core targets are TNF,AKT1,SRC,TP53,EGFR,etc.According to KEGG analysis,it is predicted that the drug will exert its therapeutic effect on diabetic cardiomyopathy mainly by intervening in cancer,MAPK,PI3K-Akt and other signaling pathways.Conclusion:Pseudostellariae Radix Pseudostellariae-Moutan peel drug pair can protect diabetic cardiomyopathy through multiple active ingredients,multiple targets,and multiple pathways,providing new ideas for the future prevention and treatment of diabetic cardiomyopathy.