胶体金法与荧光定量聚合酶链反应法在流感样呼吸道感染病原体实验室检测的对比分析

Comparative Analysis of Colloidal Gold and Fluorescent PCR Methods for Laboratory Detection of Influenza-Like Respiratory Infection Pathogens

目的:对比分析胶体金法与荧光定量聚合酶链反应(PCR)法在流感样呼吸道感染病原体实验室检测。方法:选取2023年4月本院收治的245例流感症状上呼吸道感染患者,以荧光定量PCR法检查结果为金标准,比较荧光定量PCR法、胶体金法的阳性检出率。结果:245例流感症状上呼吸道感染患者,用荧光定量PCR法检测出阳性165例,用胶体金法进行甲型流感病毒抗原检测出阳性96例,通过对比两种不同检测方法发现,荧光定量PCR法的检测灵敏度高于胶体金法,Kappa值为0.904。荧光定量PCR法对不同病毒类型的诊断阳性率均高于胶体金法。结论:荧光定量PCR检测具有检测速度快、准确度高、假阳性率低等优点,有利于提高医务人员对流感病毒的检测质量,对合理控制流感疫情具有积极的影响。

Objective:Comparative analysis of colloidal gold and fluorescent PCR methods for laboratory detection of influenza-like respiratory infection pathogens.Methods:A total of 245 patients with upper respiratory tract infections with influenza symptoms admitted to our hospital in April 2023 were selected to compare the positive detection rates of fluorescence quantitative PCR and colloidal gold methods using the results of the fluorescence quantitative PCR method as the gold standard.Results:In 245 patients with upper respiratory tract infections with influenza symptoms,165 cases were detected positively by fluorescent PCR,and 96 cases were detected positively by colloidal gold for influenza a virus antigen detection.Comparison of the two different detection methods showed that fluorescent PCR had a higher sensitivity than colloidal gold,with a Kappa value of 0.904.Fluorescent PCR had a higher diagnostic positivity rate for different virus types than colloidal gold.Conclusion:Fluorescence quantitative PCR assay has the advantages of fast detection speed,high accuracy and low false-positive rate,which is conducive to the improvement of the quality of influenza virus detection by medical personnel and has a positive impact on the rational control of influenza epidemics.