实时荧光定量PCR在血站核酸检测中的应用效果

Application of Real-Time PCR in Nucleic Acid Detection in Blood Stations

目的:观察并分析在血站核酸检测中实时荧光定量聚合酶链反应(PCR)的应用效果。方法:选取2023年1月~2023年6月17564分无偿献血者样本,都是经过乙型肝炎病毒表面抗原、丙型肝炎病毒抗体、人类免疫缺陷病毒抗体初检和复检都合格的样本,对其进行实时荧光定量PCR的核酸检测。结果:在17564无偿献血者的血液样本中,经过实时荧光定量PCR技术检测,共有25例乙型肝炎病毒脱氧核糖核酸阳性,0例丙型肝炎病毒核糖核酸和人类免疫缺陷病毒(1型)核糖核酸阳性。在临检中心举行的室间质评中,所有检验结论均正确,得分为100分。结论:在血站核酸检测中采用实时荧光定量PCR技术,可有效减短病毒检测窗口期,有助于血液安全水平的提高。

Objective:To observe and analyze the application effect of real-time quantitative PCR in nucleic acid detection in blood stations.Methods:Samsamples of 17564 from January 2023 to June 2023 were selected for HBsAg,anti-HCV,anti-HCV,anti-HIV,anti-TP,ALT and reexamination for nucleic acid detection by real-time PCR.Results:In the blood samples of 17564 voluntary blood donors,a total of 25 cases were detected as positive for hepatitis B virus deoxyribonucleic acid,and 0 cases as positive for hepatitis C virus ribonucleic acid and human immunodeficiency virus(type 1)ribonucleic acid using realtime fluorescence quantitative PCR technology.In the inter room quality assessment conducted at the clinical inspection center,all inspection conclusions were correct,with a score of 100 points.Conclusion:Real-time quantitative PCR technology used in nucleic acid detection in blood stations can effectively reduce the virus detection window period and contribute to the improvement of blood safety level.