含酚废水SAD工艺的启动及污泥特性

Start-up of SAD process treating phenol-containing wastewater and its sludge characterizations

在升流式厌氧污泥床(UASB)反应器内以厌氧氨氧化(Anammox)颗粒污泥为基础,通过先添加乙酸钠再逐步替换为苯酚的方式,启动厌氧氨氧化耦合异养反硝化(SAD)工艺;考察启动过程中耦合系统的脱氮除碳性能及颗粒污泥性状。结果表明:系统启动成功时,TN、COD及苯酚的去除率分别为84.78%、85.23%和84.09%,总氮去除负荷(NRR)及碳去除负荷(CRR)分别为0.73、0.31 kg·(m^(3)·d)^(-1);苯酚对Anammox菌的抑制作用更强,反硝化菌在Anammox颗粒污泥表面增殖;溶解性微生物产物(SMP)和胞外聚合物(EPS)含量增多,FT-IR和3D-EEM分析进一步证实,蛋白质(PN)是SMP、EPS的主要成分,色氨酸和酪氨酸类物质在抵抗苯酚毒性、维持颗粒污泥稳定中起到重要作用。

The anaerobic ammonium oxidation(Anammox) granular sludge was used as the basis to start the simultaneous anammox and denitrification(SAD) process in an upflow anaerobic sludge bed(UASB) reactor by adding sodium acetate and then replacing it with phenol step by step.The denitrification and carbon removal performance of the coupled system as well as the characteristics of the granular sludge was investigated during the startup process.The results showed that when the system was successfully started,the removal rates of TN,COD,phenol were 84.78%,85.23%,84.09%,respectively,the nitrogen removal rate(NRR) and carbon removal rate(CRR) were 0.73,0.31 kg·(m^(3)·d)^(-1),respectively.Phenol had a stronger inhibitory effect on the Anammox bacteria,the denitrifying bacteria proliferated on the surface of the granular sludge of Anammox.The content of soluble microbial products(SMP) and extracellular polymeric substances(EPS) increased.FT-IR and 3D-EEM analyses further confirmed that protein(PN) was the main components of SMP and EPS,tryptophan-like and tyrosine-like analogs played an important role in resisting phenol toxicity and maintaining the stability of granular sludge.