星点设计-效应面法优化乙醚提取万寿菊中叶黄素的工艺条件

Central Composite Design-Response Surface Method to Optimize Ether Extraction Technology Conditions of Lutein from Marigold

为优化从万寿菊鲜花中提取叶黄素的提取工艺条件,以乙醚与水的不同混合比例及振荡时间作为考察对象,提取率作为考察指标,在单因素试验基础上,采用星点设计-效应面法,通过对设计的13组试验结果进行多元线性回归分析,建立数学模型,绘出三维效应面图,优化出最佳提取条件为:乙醚与水的体积比为7.12,振荡时间为27.78 min。按照最佳工艺条件实测提取率是2.51%,与理论预测的偏差值为4.9%。该模型失拟项不显著,模型可靠,实验设计简单,具有较好的可预测性。

In order to optimize the extraction conditions of lutein from marigold flowers,the different mixing ratio of ether and water and the oscillation time were taken as the object of study,the extraction rate was taken as the index,and on the basis of single factor test,13 sets of experimental results designed were multiple linear regression analysed by the Central Composite Design-Response Surface Method.Mathematical model was established and 3D response surface map was plotted.The optimum extraction conditions were as follows:the volume ratio of ether to water was 7.12,and the oscillation time was 27.78 min.According to the optimum technological conditions,the actual extraction rate was 2.51%,and the deviation from the theoretical prediction was 4.9%.It shows that the model is reliable,simple in experimental design and has good predictability.