南酸枣叶原花青素的提取工艺优化及抗氧化活性研究

Optimization of extraction process of proanthocyanidins from Choerospondias axillaris leaves and evaluation of antioxidative activity

本试验以南酸枣叶为原料,丙酮为溶剂,采用超声辅助提取法提取南酸枣叶中的原花青素,考察提取时间、料液比、温度和丙酮浓度四个因素对南酸枣叶原花青素得率的影响,结合正交试验优化南酸枣叶原花青素的提取工艺。最后对南酸枣叶原花青素的抗氧化能力进行测定。结果表明:南酸枣叶原花青素的最佳提取工艺为超声时间8 min、料液比1:25、温度45℃、丙酮体积分数0.7。该条件下,原花青素得率为16.85 mg/g。另外,南酸枣叶原花青素有一定的抗氧化活性,其对DPPH和OH自由基的IC50分别为0.83 mg/mL和1.50 mg/mL。

The proanthocyanidins of Choerospondias axillaris leaves were extracted by ultrasonic-assisted extraction with acetone as solvent.The effects of extraction time,solid-liquid ratio,temperature and acetone concentration on the yield of proanthocyanidins in of Choerospondias axillaris leaves were investigated,and the extraction process of proanthocyanidins was optimized by orthogonal experiment.Finally,the antioxidant capacity of proanthocyanidins from Choerospondias axillaris leaves was determined.The results showed that the optimum extraction process was ultrasonic time 8 min,solid-liquid ratio 1:25,temperature 45℃,acetone volume fraction 0.7.Under the condition,the yield of proanthocyanidins reached 16.85 mg/g.The proanthocyanidins of Choerospondias axillaris leaves had certain antioxidant activity,and their IC50 to DPPH and OH free radicals were 0.83 mg/mL and 1.50 mg/mL,respectively.