夏季高温条件下鲜饲金荞麦对蛋鸡肠道微生物区系及相关基因表达的影响

Effects of Feeding Fresh Fagopyrum dibotrys on Intestinal Microflora and Related Gene Expression of Laying Hens under High Temperature in Summer

课题组前期研究发现鲜饲金荞麦可维持蛋鸡在夏季自然高温条件下的产蛋率,但具体作用机制未知,本试验拟从肠道相关基因表达和微生物区系角度探究鲜饲金荞麦对蛋鸡的作用机理。试验选取32周龄的长顺绿壳蛋鸡300只,随机分为5组(C组饲喂基础饲粮,T1、T2、T3和T4组分别饲喂添加5%、10%、15%和20%新鲜金荞麦的基础饲粮),每组5个重复,每个重复12只,试验期90 d。结果表明,T2组显著上调了蛋鸡十二指肠和回肠中的Occluding和EGF的表达量(P<0.05);试验组蛋鸡回肠中蓝细菌门(Cyanobacteria)相对丰度较高;T2组增加了蛋鸡回肠中的双歧杆菌属(益生菌)丰度和盲肠中的乳杆菌属丰度。因此,蛋鸡饲粮中添加新鲜金荞麦可能通过上调肠道中Occluding和EGF基因的表达量以及增加肠道有益菌丰度的途径缓解高温环境带来的应激效应。

The previous researches of the research group found that feeding fresh Fagopyrum dibotrys can maintain the laying rate of laying hens under natural high temperature conditions in summer,but the specific mechanism is unknown.This experiment intended to explore the mechanism of feeding fresh Fagopyrum dibotrys on laying hens from the perspective of intestinal related gene expression and microflora.30032-week-old Changshun green shell layers were randomly divided into 5 treatment groups(Group C basic diet,T1 basic diet+5%fresh Fagopyrum dibotrys,T2 basic diet+10%Fagopyrum dibotrys,T3 basic diet+15%fresh Fagopyrum dibotrys,and T4 basic diet+20%fresh Fagopyrum dibotrys),and each treatment had 5 replicates,12 layers in each replicate,and the experimental period was 90 days.The results showed:the expression of Occluding and EGF in duodenum and ileum of laying hens in T2 significantly increased(P<0.05).The abundance of bifidobacteria(probiotics)in the ileum and Lactobacillus in the cecum of laying hens of T2 were increased.Therefore,the supplementation of fresh Fagopyrum dibotrys to the diet of laying hens may act on the positive resistance of laying hens and alleviate the stress effect of high temperature environment by up-regulating the expression of Occluding and EGF genes in the intestine and increasing the abundance of beneficial bacteria in the intestine.