一种新型复合消毒剂消毒效果研究

Study on the disinfection effect of a new compound disinfectant

目的研究一种新型复合消毒剂对不同种类微生物的杀灭效果及作用机制。方法采用悬液定量杀菌试验方法,对该消毒剂的杀菌效果进行研究;采用实时荧光定量PCR和透射电镜研究消毒剂对微生物的杀灭机制。结果该消毒剂对大肠埃希菌和铜绿假单胞菌作用30 s,可全部杀灭;对金黄色葡萄球菌作用30 s,杀灭对数值>5.00;对枯草杆菌黑色变种芽孢作用60 min,杀灭对数值为1.19。复合消毒剂处理大肠埃希菌后的膜蛋白基因LptD、LptE、OmpA、OmpC表达量较对照组略有升高;复合消毒剂处理后大肠埃希菌和金黄色葡萄球菌细胞膜发生破损。结论该新型复合消毒剂能够有效杀灭细菌繁殖体,主要通过破坏细菌的细胞膜以及氧化细胞内物质达到杀灭微生物的效果。

Objective To study the efficacy and mechanism of a new compound disinfection in killing different types of microorganisms.Methods The suspension quantitative bactericidal test method was used to study the bactericidal effect of the disinfectant;real-time fluorescence quantitative PCR and transmission electron microscopy were used to study the killing mechanism of the disinfectant on microorganisms.Results The disinfectant acted on Escherichia coli and Pseudomonas aeruginosa for 30 s and could kill them all;on Staphylococcus aureus for 30 s,the killing log value>5.00;on spores of Bacillus subtilis var niger for 60 min,the killing log value was 1.19.After Escherichia coli were trented by the compound disinfectant the expression levels of membrane protein genes LptD,LptE,OmpA,OmpC were slightly increased compared to the control group;the cell membrane of Escherichia coli and Staphylococcus aureus were damaged by the compound disinfectant.Conclusion This new compound disinfectant can effectively kill bacterial propagules mainly by destroying bacterial cell membrane and oxidizing intracellular substances to kill microorganisms.