基于LC-MS/MS的鹿角方中红景天苷在慢性心力衰竭大鼠心肌组织中的分布

Distribution of salidroside in Lujiao Fang in the myocardial tissues of rats with chronic heart failure based on LC-MS/MS

目的分析鹿角方中红景天苷在慢性心力衰竭大鼠心肌组织中的分布。方法45只SPF级雄性Wistar大鼠,6~8周龄,采用简单随机抽样法选取8只作为假手术组,假手术组大鼠只暴露腹主动脉不结扎;其余大鼠行腹主动脉缩窄手术,以血管结扎处明显缩窄示手术成功,术后将存活的32只模型大鼠按体重进行区组随机分组,分为模型组(n=8)、鹿角方低剂量组(1.100 g/kg,n=8)、鹿角方中剂量组(2.200 g/kg,n=8)、鹿角方高剂量组(4.400 g/kg,n=8),药物灌胃干预30周。末次灌胃给药2 h后麻醉大鼠,腹主动脉取血,采集心肌组织,LC-MS/MS法分析不同剂量鹿角方组红景天苷在大鼠左、右心室壁的分布。结果红景天苷在1.195~1195.000 ng/ml范围内线性关系良好(r>0.999),精密度RSD%<15%,准确度为90.817%~106.500%,相对回收率为95.222%~100.814%,基质效应RSD%<15%,样品冰浴放置1 h、前处理后室温放置6 h及自动进样器放置48 h内红景天苷的稳定性良好。红景天苷在右心室壁含量与给药剂量线性拟合值R2为0.983,在中、高剂量鹿角方组中红景天苷在左心室壁的含量分别为右心室壁含量的(47.383±12.156)%、(43.805±9.644)%。结论建立的分析方法具有可行性,红景天苷在慢性心力衰竭大鼠左、右心室分布存在差异。

Objective To analyze the distribution of salidroside in Lujiao Fang in myocardial tissues of rats with chronic heart failure.Methods A total of 45 SPF male Wistar rats,aged 6-8 weeks,were selected.A simple random sampling method was used to select eight rats as sham operation group.In sham operation group,only the abdominal aorta was exposed without ligature;the left rats underwent abdominal aortic coarctation,and the obvious coarctation at the vessel ligation site indicated successful operation.After operation,according to the body weight of 32 surviving rats,model rats were randomly divided into the model group(n=8),low-dose Lujiao Fang group(1.100 g/kg,n=8),medium-dose Lujiao Fang group(2.200 g/kg,n=8),and high-dose Lujiao Fang group(4.400 g/kg,n=8),and given intragastric intervention of drug for 30 weeks.The rats were anesthetized,blood was collected from the abdominal aorta,and the hearts were collected 2 h after the last intragastric administration.LC-MS/MS method was used to analyze the distribution of salidroside in left and right ventricular wall of rats with different doses.Results Salidroside had a good linear relationship in the range of 1.195-1195.000 ng/ml(r>0.999),precision RSD%<15%,accuracy was 90.817%-106.500%,relative recovery was 95.222%-100.814%,substrate effect RSD%<15%.The stability of salidroside was good after the sample was placed in ice bath for 1 h,at room temperature for 6 h after pretreatment,and 48 h after automatic sampler.The linear fitting value R2 of salidroside content in right ventricular wall and dose was 0.983.The content of salidroside in left ventricular wall was(47.383±12.156)%and(43.805±9.644)%of right ventricular wall,respectively.Conclusion The established analytical method is feasible.The distribution of salidroside in left and right ventricular wall of chronic heart failure rats is different.