内蒙古岱海湖的细菌多样性分析

Study on microbial diversity in Daihai lake,Inner Mongolia

通过高通量测序对内蒙古岱海湖的沉积物与水样进行了细菌群落结构特征和细菌多样性研究。结果显示:沉积物中细菌OTUs(Operational Taxonomic Units)为3253;水体中细菌的OTUs为640,沉积物较水体中细菌群落更加多样化。岱海湖沉积物中细菌种类隶属于58门,782属,其优势种群为变形菌门(Proteobacteria,49%)、拟杆菌门(Bacteroidetes,11%)、绿弯菌门(Chloroflexi,8%)和厚壁菌门(Firmicutes,6%);水体中细菌隶属于29门,318属,优势种群为放线菌门(Actinobacteria,45%)、变形菌门(26%)、拟杆菌门(Bacteroidetes,16%)和柔膜菌门(Tenericutes,13%)。ANOSIM检验显示,岱海湖沉积物细菌群落与水体细菌群落结构呈现显著性差异。盐度是影响湖泊中细菌多样性的主要制约因素。较高丰度的变形菌门、拟杆菌门、蓝藻细菌、浮霉菌门综合显示水体出现恶化趋势。

Microbial community and diversity of water and sediment samples were investigated by the 16S rRNA gene based on a high-throughput sequencing platform.Results showed that the species annotation OTU(Operational taxonomic unit)numbers of Daihai water and sediments were 3253 and 640 respectively,and the bacterial community in the sediments are more diverse than that in the water body.The bacteria species in the sediments of Daihai Lake belong to 58 phyla(782 genera),and the dominant groups are Proteobacteria(49%),Bacteroidetes(11),Chloroflexi(8%)and Firmicutes(6%).The bacteria in the water are assigned to 29 phyla and 318 genera,and the dominant populations are Actinobacteria(45%),Proteobacteria(26%),Bacteroidetes(16%)and Tenericutes(13%).ANOSIM test showed that there was a significant difference in the bacterial community structure between the sediment and the water samples of Daihai Lake.Salinity is a major constraint to the bacterial diversity in lakes.The higher abundance of Proteobacteria,Bacteroidetes,cyanobacteria,Planctomycetes,which showed the deterioration trend of water samples.