精准放疗下C57BL/6J鼠放射性肝损伤模型的建立

Establishment of a RILD model in C57BL/6J mice treated with precise radiotherapy

目的 为探寻放射性肝损伤(RILD)分子致病机制提供存活稳定的动物研究模型。方法 将90只C57BL/6J鼠分为对照组、20 Gy、25 Gy、30 Gy和35 Gy辐射组,于辐射后4周处死小鼠,检测小鼠肝脏血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)水平,对小鼠肝脏病理组织做HE染色,于辐射36周后进行Masson染色。结果 与对照组相比,30 Gy和35 Gy辐射组小鼠致死率高,20 Gy和25 Gy辐射组小鼠体重均出现明显下降。20 Gy辐射组小鼠ALT水平(相对于对照组)显著升高。25 Gy辐射组小鼠AST和ALT水平(相对于对照组)升高。20 Gy及25 Gy辐射组小鼠肝脏大体观察未见明显变化,但病理检测表明,20 Gy与25 Gy处方剂量均诱导小鼠肝脏产生放射性肝损伤。结论 在直线加速器下构建了稳定可靠的RILD小鼠模型,在直线加速器下使用SBRT技术构建的RILD的小鼠模型对探索RILD疾病具有重大研究意义。

Objective To provide a reliable and stable animal model for investigating the molecular pathogenesis of radiation-induced liver disease(RILD).Methods Ninety C57BL/6J mice were divided into control,20 Gy,25 Gy,30 Gy and 35 Gy radiation groups.The mice were executed at 4 weeks after radiation and the levels of alanine aminotransferase,aspartate aminotransferase,and alkaline phosphatase in the liver serum were measured.HE staining was performed on the pathological liver tissues.Masson staining was performed at 36 weeks after radiation.Results Compared with the control group,the fatality rate was higher in the 30 and 35 Gy radiation groups,and the body weight significantly decreased in the 20 and 25 Gy radiation groups.Compared with the control group,alanine aminotransferase significantly increased in mice exposed to 20 Gy,while aspartate aminotransferase and alanine aminotransferase increased in mice exposed to 25 Gy.No significant changes were observed in the livers of the mice in the 20 and 25 Gy radiation groups,but pathological examination showed liver damage induced by both 20 and 25 Gy radiation.Conclusion A stable and reliable mouse model of RILD was constructed for treatment with linear accelerator.The mouse model of RILD constructed for stereotactic body radiation therapy using linear accelerator has significant research implications for the exploration of RILD.