橘小实蝇卵引诱其雌虫聚集和产卵研究

Bactrocera dorsalis Females Attracted to Their Eggs for Further Oviposition

橘小实蝇雌虫有聚集产卵的习性,为阐释橘小实蝇的聚集产卵行为,本文研究橘小实蝇卵引诱雌虫聚集和产卵,探究卵引诱不同橘小实蝇种群的不同日龄和不同交配状态雌虫的产卵效果,以及卵与寄主果实引诱雌虫聚集和产卵的差异。结果表明,在引诱2 h时,橘小实蝇卵的雌虫引诱率为36.00%,显著高于对照(12.00%),雄虫引诱率为0.67%,显著低于对照;不同卵量引诱雌虫时,0.4g卵对实验室种群雌虫的引诱率最高,达到47.67%,1.2 g卵对野生种群雌虫的引诱率最高,达到13.67%;不同卵量引诱雌虫产卵时,0.8 g卵引诱实验室种群的产卵数最多,为762.00粒,1.0 g卵引诱安顺种群的产卵数最多,为790.83粒;卵引诱不同日龄雌虫产卵时,实验室种群在15 d的产卵数为2945.00粒,安顺种群在12 d的产卵数为2530.50粒;卵引诱橘小实蝇实验室种群、遗传区性品系种群、安顺种群和福州种群已交配雌虫的产卵数明显多于未交配雌虫;在选择性和非选择性测试中,卵引诱雌虫产624.33粒和844.00粒卵,均显著高于番石榴、杨桃和鲜橙果实。本研究明确了橘小实蝇卵能引诱雌虫聚集和产卵,且产卵数高于寄主果实。

Bactrocera dorsalis Hendel females have the habit of gathering together to lay eggs.To elucidate the aggregation and oviposition behavior of the fruit fly,this study investigated the role of B.dorsalis eggs in attracting the females to gather and lay eggs,and explored the effects of the attraction on B.dorsalis females of different ages and mating status and the differences between eggs and host fruits in attracting the females to gather and lay eggs.The results showed that B.dorsalis females were significantly more attracted to their eggs(36.0%)than to the control(12.0%)within 2 h;in contrast,males were attracted significantly less to their eggs(0.67%)within 2 h than to the control.The attraction of the females differed between laboratory and wild B.dorsalis populations,being the highest of 47.67%at 0.4 g eggs for the laboratory population and 13.67%at 1.2 g eggs for the wild population.We detected an effect of egg amount on the induction of oviposition,0.8 g eggs induced the largest oviposition(762.0 eggs)in the laboratory population and 1.0 g eggs induced the largest oviposition(790.8 eggs)in the Anshun population.When tested against females of different ages,the eggs induced the laboratory population to deposit 2945.0 eggs on 15 days and the Anshun population to deposit 2530.5 eggs on 12 days.The eggs laid by mated females in the laboratory populations,genetic strains,Anshun populations,and Fuzhou populations were significantly more than that by unmated females.In choice and no-choice tests,eggs induced B.dorsalis oviposition of 624.33 and 844.00 eggs,respectively,which were significantly higher than those induced by fruits of Psidium guajava,Averrhoa carambola and Citrus sinensis.These results indicate that B.dorsalis eggs can attract the B.dorsalis females to gather and induce oviposition,and the oviposition induction is higher than that of the host fruit.