淡紫拟青霉M-1羽碳双基双层微胶囊制备及工艺优化

Preparation and Process Optimization of Feather Carbon-based Bilayer Microcapsules of Paecilomyces lilacinus M-1

淡紫拟青霉在线虫生物防治上表现出巨大的潜力,但现今产品货架期短、土壤定殖率低及生防效果不稳定限制其大规模市场应用。为解决上述问题,建立淡紫拟青霉M-1微胶囊最佳包埋工艺,本研究发展了喷雾法结合钙离子固化法,通过单因素及响应面试验优化确定淡紫拟青霉M-1微胶囊制备的最佳工艺条件,得到最佳工艺参数为:海藻酸钠添加量3.183%、菌粉与海藻酸钠溶液的质量比1.71:100、膨化鸡羽毛粉添加量4%、活性碳添加量4%、进料流量为3.7 L/h、CaCl_(2)浓度为3%、壳聚糖浓度为0.858%,在此优化条件下制备的微胶囊于不同环境下存储,微胶囊剂型菌株稳定性均优于粉剂剂型菌株,室温下存储12个月,仍保存20%以上活菌数。土壤定殖试验表明,不同淡紫拟青霉M-1剂型土壤定殖效果为:羽碳双基双层微胶囊>碳基双层微胶囊>粉剂。由此可见,该工艺解决淡紫拟青霉大规模市场应用的瓶颈问题,具有较高的应用价值。

Paecilomyces lilacinus has shown great potential in biological control of nematodes.However,its large-scale market application is limited by short product shelf life,low soil colonization rate,and unstable biocontrol effect.To solve these problems,this study developed a spray method combined with calcium ion solidification method to establish the best embedding process for P.lilacinus M-1 microcapsules.The optimal process conditions for preparing P.lilacinus M-1 microcapsules were determined by single-factor and response surface experiments through optimization.The best process parameters were:sodium alginate addition 3.183%,mass ratio of spores to sodium alginate solution 1.71:100,expanded feather powder addition 4%,activated carbon addition 4%,feed flow rate 3.7 L/h,CaCl_(2) concentration 3%,chitosan concentration 0.858%.Under this optimized condition,the stability of microcapsule strains in different environments was better than that of powder strains.Microcapsules are stored at room temperature for 12 months and still retain more than 20%of the total viable count.Soil colonization experiments showed that the soil colonization effects of different P.lilacinus M-1 formulations were as follows:feather carbon-based bilayer microcapsules>carbon-based bilayer microcapsules>powder.Therefore,this process solves the bottleneck problem of large-scale market application of P.lilacinus and has high application value.