MdSAUR71和MdSAUR72基因调控苹果果实花色素苷积累的验证

Functional validation of MdSAUR71 and MdSAUR72 in regulating anthocyanin accumulation in apple fruit

【目的】为探明MdSAUR71和MdSAUR72基因与苹果花色素苷积累的关系。【方法】选取了‘王林’(Malus domestica)愈伤组织作为试验材料,通过构建过表达载体及稳定转化愈伤组织操作,获得MdSAUR71和MdSAUR72大量表达的转基因愈伤组织。【结果】转基因愈伤组织在低温光照下培养,与对照愈伤组织相比呈现明显深红色,推测是由于花色素苷积累所致。进一步通过实时荧光定量PCR (Real Time-quantitative PCR)检测,发现与对照愈伤组织相比,MdSAUR71和MdSAUR72及花色素苷生物合成基因在转基因愈伤组织中的转录水平明显增加。【结论】MdSAUR71和MdSAUR72能够通过调控花色素苷生物合成基因的表达水平,参与苹果果实花色素苷积累。

[Objective]In order to understand the regulation mechanism of MdSAUR71 and MdSAUR72 on apple anthocyanin biosynthesis,[Methods]the apple calli of Malus domestica‘Orin’was selected as the experimental materials.MdSAUR71 and MdSAUR72 were constructed into overexpression vectors and transformed into apple calli successfully.[Results]After 15 days of light and low temperature cultivated,the transgenic apple calli were displayed red color,which suggested that anthocyanin were accumulated in transgenic apple calli.Compared with wild type control,the expression of MdSAUR71 and MdSAUR72 genes and anthocyanin biosynthesis genes were significantly increased in transgenic calli by real-time quantitative PCR.[Conclusion]The results showed that MdSAUR71 and MdSAUR 72 are participate in anthocyanin biosynthesis by coordinating the transcription of anthocyanin biosynthesis genes.