基于SSR构建中国主栽草莓品种指纹图谱

The fingerprint of main strawberry varieties in China was constructed based on SSR molecular marker

【目的】利用SSR分子标记构建中国主栽草莓品种的DNA指纹图谱,为保护育种者权益和实现草莓资源的高效鉴定提供基础数据。【方法】以中国目前主栽的草莓品种(70个)为材料,采用荧光毛细管电泳技术对28个SSR分子标记进行评估,利用筛选后的高效SSR标记构建草莓品种的DNA指纹图谱。【结果】从28对引物中选出了5对多态性较高、重复性较好的核心引物。核心引物共检测出40个等位位点,平均每对引物检测出8个等位位点;共检测到142个带型,平均每对引物检测出28.4个带型;各标记的多态信息含量(PIC)为0.79~0.87,平均PIC值为0.83;聚类分析结果表明,草莓品种之间的相似系数范围为0.65~0.97。【结论】成功构建了具有唯一对应关系的70个草莓品种的DNA指纹图谱,为未来草莓品种的资源保护和利用推广提供数据支撑。

[Objective]Constructing the DNA fingerprints of the main strawberry varieties in China using SSR molecular markers to provide fundamental data for the protection of the rights and interests of breeders and the efficient identification of strawberry resources.[Methods]Using the current mian strawberry varieties(70 in total)currently planted in China as materials,28 SSR molecular markers were evaluated by fluorescence capillary electrophoresis technology,and DNA fingerprints of strawberry varieties were constructed by using screened high-efficiency SSR markers.[Results]Five pairs of core primers with high polymorphism and good repeatability were selected from the 28 pairs of primers.A total of 40 alleles were detected by the core primers,with an average of 8 alleles per primer pair.A total of 142 bands were detected,averaging 28.4 bands per primer pair.The polymorphic information content(PIC)of the markers ranged from 0.79 to 0.87,with an average PIC value of 0.83.Celuster analysis-results showed that the similarity coefficients between strawberry varietie ranged 0.65 to 0.97.[Conclusion]The DNA fingerprints of 70 strawberry varieties with unique correspondence have been successfully constructed,which will provide data support for resource conservation and use promotion of strawberry varieties in the future.