猪源SLPI对小鼠皮肤损伤愈合的效果

Exploration of the effect of porcine secretory leukocyte protease inhibitor on skin injury healing in mice

【目的】探究猪源分泌性白细胞蛋白酶抑制因子(Secretory Leukocyte Protease Inhibitor, SLPI)对皮肤创伤愈合过程中的治疗效果,以开发其潜在的使用价值。【方法】使用重组表达的猪源SLPI处理小鼠创伤,筛选蛋白的最佳作用浓度,再以此浓度探索其抗损伤愈合情况。设置创伤组、SLPI处理组及阳性药对照组(阳性药物为百灵金方抑菌液),观察不同时间大体病理变化与显微病理变化,对创伤愈合进行临床评分,来评判SLPI对伤口恢复的影响作用。【结果】结果显示,SLPI最佳作用浓度为0.25 mg/mL。三组小鼠创伤溃疡面随时间延长均逐渐减小;与创伤组相比,从第3天开始,SLPI处理组病理打分均低于创伤组(P<0.05或P<0.01),而与阳性药组表现接近(P<0.05);在第7天,阳性药组和SLPI组均可观察到毛囊生长,但创伤组变化不明显。显微病理变化显示,SLPI组和阳性药组在第5天可观察到表皮层和皮脂腺,在第7天成纤维细胞数量明显较多,炎性细胞浸润减少。【结论】重组表达的猪源SLPI对小鼠皮肤创伤愈合有一定效果,为开发针对猪体皮肤损伤有效药物提供了试验数据。

[Objective]To investigate the therapeutic effect of porcine secreted leukocyte protease inhibitor(SLPI)on skin wound healing and explore its potential value.[Methods]This study used recombinant pig derived SLPI to treat mouse wounds,screened the optimal concentration of the protein,and then explored its anti injury healing effect at this concentration;The experiment was divided into trauma group,SLPI treatment group,and positive drug control group(Positive drug represents Bailingjinfangyijunye).The gross pathological changes of mouse wounds at different times and microscopic pathological changes were observed,and the clinical score of wound healing were used to evaluate the effect of SLPI on wound recovery.[Results]The results showed that the optimal concentration of SLPI was 0.25 mg/mL.The ulcerated area of the wound gradually decreased over time in all three groups of mice.Compared with the trauma group,from day 3 onwards,the pathological scores of the SLPI treatment group were lower than those of the trauma group(P<0.05 or P<0.01),and the performance was similar to that of the positive drug group.At day 7,both the positive drug control group and SLPI group showed hair follicle growth,while changes in the injury group were not significant.Microscopic pathological changes showed that compared to the blank control group,the SLPI group and positive drug control group displayed epidermal layers and sebaceous glands on day 5,and on day 7,a significantly higher number of fibroblasts and reduced inflammatory cell infiltration were observed.[Conclusion]In summary,the recombinant expression of pig derived SLPI has a certain effect on mouse skin wound healing,providing experimental data for the development of effective drugs targeting pig skin injury.