基于高效液相色谱法的苦参指纹图谱研究

Study on fingerprint of Sophorae flavescentis based on high performance liquid chromatography

【目的】旨在建立苦参高效液相色谱指纹图谱。【方法】采用Waters Atlantis■T3色谱柱(250 mm×4.6 mm, 5.0μm),流动相为0.01 mol/L乙酸铵(0.045%浓氨水)和乙腈,流速1.0 mL/min,梯度洗脱,色谱柱温度30℃,检测波长225 nm。检测12批苦参样品,建立指纹图谱,进行相似度评价、聚类分析和主成分分析。【结果】建立了苦参的高效液相色谱指纹图谱,苦参样品的相似度在0.940~0.998之间,共标定出23个共有峰,并通过标准品确认了其中5个,分别为氧化苦参碱、氧化槐果碱、槐定碱、苦参碱和槐果碱。聚类分析将12批苦参样品分为4类,主成分分析后23个共有峰缩减为4个主成分。【结论】该方法快速可靠,稳定性和重复性好,可用于苦参的质量控制。

[Objective]To establish fingerprints of Sophorae flavescentis Radix by high performance liquid chromatography.[Methods]The chromatographic separation was performed on a Waters Atlantis ■ T3(250 mm×4.6 mm,5μm)analytical column.The mobile phases were 0.01 mol/L ammonium acetate with 0.045%ammonium hydroxide and acetonitrile at a flow rate of 1.0 mL/min with gradient elution.The column temperature was 30℃and the detection wavelength was 225 nm.12 batches of samples were detected,and the fingerprint was established.Similarity evaluation,cluster analysis and principal component analysis were conducted.[Results]The results showed that high performance liquid chromatography fingerprints for Sophorae flavescentis Radix were successfully established,and similarities of their fingerprints were between 0.940 and 0.998.12 batches of Sophorae flavescentis Radix possessed 23 common peaks and 5 common peaks could be identified using standard products,which were oxymatrine,oxysophocarpine,sophoridine,matrine and sophocarpine,respectively.They were divided into 4 groups by cluster analysis,and 23 common peaks were reduced into 4 principal components after principal component analysis.[Conclusion]The method is fast,reliable,stable and reproducible,and can be used for quality control of Sophorae flavescentis Radix.