当归根际丛枝菌根真菌群落及其影响因子分析

Comparison of Community Characteristics and Influencing Factor Analysis of Arbuscular Mycorrhizal Fungal Communities in Rhizospheric Soil of Angelica sinensis

分析丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)菌群在当归生长过程中的变化,以期为AMF在当归提质增效中的应用提供一定的研究基础。基于Illumina Miseq高通量测序技术,测定当归叶丛期、根膨大期和成药期根际土壤的AMF多样性和菌群组成。同时测定根际土壤理化特性和重金属镉和砷含量,并分析AMF菌群对土壤因子的响应。结果显示:随着当归的生长,当归根际土壤AMF菌群的α多样性增加,β多样性存在差异;当归根际AMF的优势菌属为球囊霉属Glomus_f_Glomeraceae和多孢囊霉属Diversispora;在当归的生长阶段,多孢囊霉属的丰度逐渐降低,而球囊霉属的丰度逐渐增加成为绝对优势菌,仅Unclassified_o_Diversisporales属在当归不同生长期的差异显著(p<0.05);土壤pH值、电导率及重金属镉和砷共同影响当归根际AMF的多样性和菌群组成。以上结果表明AMF多样性和菌群组成在当归的不同生长期具有明显的变化,土壤因子是AMF多样性和菌群组成变化的重要驱动因素,这些发现将有助于揭示当归生长与AMF的相互关系和机制,为当归的生态种植和提质增效提供理论参考依据。

In this paper,the variation of arbuscular mycorrhizal fungi(AMF)was analyzed during the growth process of Angelica sinensis(A.sinensis),to provide a scientific basis for the application of AMF in improving the quality and efficiency of A.sinensis.The Illumina Miseq sequencing was used to determine the diversity and microbial com-munity composition of AMF at the leaf cluster stage,the root expansion stage and the medicinal stage of A.sinensis,as well as the physicochemical characteristics of every stage rhizosphere soil and contents of heavy metals cadmium and arsenic,were measured.Furthermore,the response of the AMF microbial community to soil factors was ana-lyzed.The results illustrated that with the growth of A.sinensis,theα-diversity of AMF in rhizosphere soil in-creased,and there were also distinct differences inβ-diversity.The dominant bacteria of AMF in rhizospheric soil of A.sinensis were Glomus_f_Glomeraceae and Diversispora at the genus level.With the growth of A.sinensis,the a-bundance of Diversispora gradually decreased,while that of Glomus_f_Glomeraceae increased to become absolute dominant genus.Whereas,only Unclassified_o_Diversisporales displayed significant differences in different growth stages of A.sinensis(p<0.05).The diversity and the microbial community of AMF were affected by the soil factors about soil pH,electrical conductivity,and heavy metals such as cadmium and arsenic.These results showed that AMF diversity and flora composition had marked changes during different growth stages of A.sinensis,and soil fac-tors were important driving forces for variations of AMF diversity and microbial community composition.These find-ings will be likely to reveal the interrelationship and mechanism between AMF and the growth of A.sinensis,provide theoretical reference for ecological planting and the quality and efficiency improvement of A.sinensis.