临沂地区草莓叶枯病病原菌的分离鉴定及其生物学特性研究

Isolation,Identification and Biological Characteristics of the Pathogen of Strawberry Leaf Blight in Linyi

为明确山东临沂地区草莓叶枯病的致病菌类型及其生物学特性。采用组织分离法和柯赫氏法则,对山东省临沭县草莓种植园的草莓叶枯样品进行病原菌的分离、鉴定以及致病性回接试验,并对其进行生物学特性研究。分离出一株草莓叶枯致病菌CM1,经形态学和分子生物学鉴定为棒状拟盘多毛孢菌(Neopestalotiopsis clavispora),该菌株可导致草莓叶片出现黑褐色病斑,后期产生墨水状黏稠小黑点,该菌株生物学特性研究表明,该菌株最适生长条件:温度25℃,pH为6,蔗糖作为碳源,氮源为牛肉膏或蛋白胨。该试验首次报道了山东临沂地区棒状拟盘多毛孢菌对草莓叶片的致病能力,为今后草莓叶枯病的病原学研究提供了依据。

To clarify the pathogenic bacteria types and biological characteristics of strawberry leaf blight in Linyi,Shandong Province.Using tissue isolation method and Koch's rule,the pathogenic bacteria were isolated,identified and pathogenicity of strawberry leaf blight samples from the strawberry plantation in Linshu County,Shandong Province,and the biological characteristics were studied.A pathogenic bacteria CM1 of strawberry leaf blight was isolated.It was identified as Neopestalotiopsis clavispora,which could cause black-brown spots on strawberry leaves and produce ink-like thick small black spots in the later stage.The biological characteristics of the strain showed that the optimum growth temperature of the strain was 25℃,pH was 6,the carbon source was sucrose,and the nitrogen source was beef extract or peptone.This experiment reported for the first time the pathogenicity of Neopestalotiopsis clavispora to strawberry leaves in Linyi,Shandong Province,providing a basis for the future study of the etiology of strawberry leaf blight.