摘要
Dear editor,Functional genomics studies of mosquito vectors are crucial for understanding their physiological behavior,pathogen transmission,and evolution,and for providing essential molecular targets for genetic control(Severson&Behura,201l;Alphey,2014;Ruzzante et al.,2019;Hong et al.,2022).The main methods involve clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene editing via embryo microinjection(Criscione et al.,2015;Kistler et al.,2015).
基金
This work was supported by the National Natural Science Foundation of China(Nos.31772527,31872262)
the Natural Science Foundation Project of Chongqing(No.CSTB2022NSCQ-MSX1355)
the Scientific and Technological Research Program of Chongqing Municipal Education Commission(KJZDK202200507,KJQN202200533)
the BAYU scholar program(YS2019027)
the Venture and Innovation Support Program for Chongqing Overseas Returnees(No.cx2022052)
the Graduate Research and Innovation Foundation of Chongqing under Grant(CYS23399).