急性心肌梗死患者两种血清因子水平及临床意义

Serum levels of SOX6 and PTEN and their clinical significance in patients with acute myocardial infarction

目的探讨Y染色体性别决定区相关高迁移率组盒蛋白6(SOX6)、第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)在急性心肌梗死(AMI)患者血清中的表达及临床意义。方法选取2021年1月至2022年3月淄博市第一医院和淄博市中心医院收治的AMI患者100例为研究组,根据主要不良心血管事件(MACE)发生情况将患者分为MACE组52例和非MACE组48例,选取同期于淄博市第一医院和淄博市中心医院进行健康体检的志愿者110例为对照组。检测血清PTEN和SOX6水平,用Pearson相关性分析AMI患者血清PTEN和SOX6与临床指标的相关性,用ROC曲线分析PTEN和SOX6水平对AMI及预后的诊断价值。结果与对照组比较,研究组血清SOX6 mRNA水平显著降低(0.69±0.14 vs 1.03±0.16,P<0.01),血清PTEN mRNA水平显著升高(1.56±0.15 vs 1.05±0.08,P<0.01)。与非MACE组比较,MACE组血清SOX6 mRNA水平显著降低(0.61±0.15 vs 0.78±0.13,P<0.01),血清PTEN mRNA水平显著升高(1.74±0.18 vs 1.37±0.12,P<0.01)。Pearson相关性分析显示,AMI患者血清PTEN水平与cTnI、CK-MB、Gensini评分呈正相关,血清SOX6水平与cTnI、CK-MB、Gensini评分呈负相关(P<0.01)。ROC曲线分析显示,血清SOX6和PTEN联合诊断AMI的曲线下面积为0.932(95%CI:0.889~0.962),二者联合预测AMI患者发生MACE的曲线下面积为0.933(95%CI:0.866~0.974)。结论AMI患者血清中SOX6水平下调,PTEN水平上调,二者联合检测有助于诊断AMI及预测MACE。

Objective To investigate the expression and clinical significance of SRY-box transcription factor 6(SOX6)and protein tyrosine phosphatase gene(PTEN)in patients with acute myocardial infarction(AMI).Methods A total of 100 AMI patients admitted to Zibo First Hospital and Zibo Central Hospital from January 2021 to March 2022 were enrolled as the study group,and according to the occurrence of major adverse cardiovascular events(MACE),they were grouped into MACE subgroup(52 cases)and non-MACE subgroup(48 cases).Another 110 volunteers who taking physical examination in above 2 hospitals during the same period were subjected as the control group.The levels of PTEN and SOX6 in the serum were detected,and Pearson correlation analysis was performed to investigate the correlation of serum PTEN and SOX6 levels with clinical indicators.ROC curve was drawn to assess the diagnostic value of PTEN and SOX6 levels for diagnosis and prognosis of AMI.Results The study group had significantly decreased serum mRNA level of SOX6(0.69±0.14 vs 1.03±0.16,P<0.01)and increased serum mRNA level of PTEN(1.56±0.15 vs 1.05±0.08,P<0.01)than the control group.Similar results were seen in the MACE subgroup than the non-MACE subgroup(SOX6:0.61±0.15 vs 0.78±0.13,P<0.01;PTEN:1.74±0.18 vs 1.37±0.12,P<0.01).Pearson correlation analysis showed that the serum level of PTEN was positively,and that of SOX6 was negatively correlated with cTnI,CK-MB and Gensini score(P<0.01).ROC curve analysis indicated that the AUC value of combined serum SOX6 and PTEN levels for diagnosis of AMI was 0.932(95%CI:0.889-0.962),and that for predicting MACE was 0.933(95%CI:0.866-0.974).Conclusion The serum level of SOX6 is down-regulated,and that of PTEN was up-regulated in AMI patients.Their combined detection is helpful for diagnosis of AMI and prediction of MACE.