人参皂苷Rg1对小鼠生精改善作用的研究

Study on Improving Effect of Ginsenoside Rg1 on Spermatogenesis in Mice

目的研究人参皂苷Rg1对邻苯二甲酸二丁酯(dibutyl phthalate,DBP)诱导的生精损伤小鼠的保护作用,为天然生精药物开发提供理论依据。方法将30只C57BL/6雄性小鼠随机分为三组,对照组、DBP组(400 mg/kg)、Rg1+DBP组(Rg120 mg/kg,DBP 400 mg/kg),连续给药35 d后,处死小鼠,收集血清、睾丸、附睾,对精子密度、活力及畸形率进行检测和计算,采用酶联免疫吸附试验检测血清睾酮(testosterone,T)、促卵泡激素(follicle-stimulating Hormone,FSH)和促黄体生成激素(luteinizing hormone,LH)的水平,测定小鼠睾丸组织超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和丙二醛(malonic dialdehyde,MDA)含量水平。结果与对照组相比,DBP组小鼠精子活力及密度明显降低(P<0.01),精子畸形率没有明显改变;血清中T、LH显著降低,FSH明显升高(P<0.01);睾丸组织GSH-Px、SOD水平明显下降,MDA含量升高(P<0.01)。与DBP组相比,DBP+Rg1组精子活力及密度明显增加(P<0.01),精子畸形率没有明显改变;血清中T、LH显著升高,FSH明显降低(P<0.01);睾丸组织GSHPx、SOD水平明显升高,MDA含量明显下降(P<0.01)。结论人参皂苷Rg1具有改善DBP诱导的生殖功能损伤的作用,机制可能与人参皂苷Rg1抗氧化损伤抗作用有关。

Objective To study the protective effect of ginsenoside Rg1 on dibutyl phthalate(DBP)-induced spermatogenic injury in mice and to provide a theoretical basis for the development of natural spermatogenic drugs.Methods Thirty C57BL/6 male mice were ran⁃domly divided into three groups:control group,DBP group(400 mg/kg)and Rg1+DBP group(Rg120 mg/kg,DBP 400 mg/kg).After con⁃tinuous administration for 35 days,the mice were killed,and serum,testis and epididymis were collected.Sperm density,motility and mal⁃formation rate were detected and calculated,and levels of testosterone(T),follicle-stimulating hormone(FSH)and luteinizing hormone(LH)in serum were detected by enzymelinked immunosorbent assay.The contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in mouse testis were determined.Results Compared with the control group,the sperm motility and density in the DBP group were significantly decreased(P<0.01),and the sperm malformation rate was not significantly changed.T and LH in serum were significantly decreased,and FSH in serum was significantly increased(P<0.01).The levels of GSH-Px and SOD in testicular tissue were significantly decreased,and the content of MDA was increased(P<0.01).Compared with the DBP group,sperm motility and density in the DBP+Rg1 group were significantly increased(P<0.01),but the sperm malformation rate was not significantly changed.T and LH in serum were significantly increased,and FSH in serum was significantly decreased(P<0.01).The levels of GSH-Px and SOD in testicular tissue were significantly increased,while the content of MDA was significantly decreased(P<0.01).Conclusion Ginsenoside Rg1 could improve DBP-induced reproductive function injury,and the mechanism may be related to the antioxidant effect of ginsenoside Rg1.