粉唑醇对假禾谷镰刀菌的抑菌活性及田间防效

Antifungal Activity and Field Control Effect of Flutriafol on Fusarium pseudograminearum

【目的】探明粉唑醇对小麦茎基腐病的防控效果,为粉唑醇在小麦安全生产上的应用提供依据。【方法】通过菌丝生长速率法测定1%粉唑醇颗粒剂对小麦茎基腐病致病菌假禾谷镰刀菌18-HX-16、19-HA-4、20-XX-13等10个菌株的室内毒力,每个菌株设置3次重复,培养48 h后计算粉唑醇对不同菌株的毒力回归方程及EC_(50)值,分析粉唑醇对假禾谷镰刀菌的抑菌活性;采用田间随机小区试验法,1%粉唑醇颗粒剂设置3个施药量(20 g/667m^(2)、30 g/667m^(2)、50 g/667m^(2))拌种进行田间防效试验。【结果】粉唑醇对供试假禾谷镰刀菌的EC_(50)在0.050~0.894μg/mL,平均为0.400μg/mL;1%粉唑醇颗粒剂对小麦茎基腐病的田间防效在49.98%~74.59%,其中,1%粉唑醇颗粒剂施药量为50 g/667m2时,小麦茎基腐病的发病率低至16.00%、病情指数低至5.87,防效高达74.59%,显著优于对照药剂(1%噁霉灵颗粒剂)的防治效果。【结论】粉唑醇对假禾谷镰刀菌具有较高的抑菌活性,对田间小麦茎基腐病有较好的防控效果,1%粉唑醇颗粒剂以50 g/667m2用量与复合肥混匀施用,对小麦茎基腐病的防效最好。

【Objective】The prevention and control effects of flutriafol on Fusarium crown rot(FCR)were explored,which provided a basis for the application of flutriafol in wheat production.【Method】The laboratory toxicity of 1%flutriafol granules to 10 isolates of Fusarium pseudograminearum,such as 18-HX-16,19-HA-4 and 20-XX-13,was determined by mycelial growth rate method.There were three replicates in each isolate.After 48 h of culture,the toxicity regression equation and EC_(50) value of flutriafol to different isolates were calculated,and the antifungal activity of flutriafol to F.pseudograminearum was analyzed.The field random plot test method was used to set up three application rates(20 g/667 m^(2),30 g/667m^(2),50 g/667m^(2))of 1% flutriafol granules with seed dressing for field control effect test.【Result】The EC_(50) values of flutriafol against F.pseudograminearum ranged from 0.050 to 0.894μg/mL,with an average of 0.400μg/mL.The field control effect of 1%flutriafol granules on FCR was 49.98%—74.59%.When the dosage of 1%flutriafol granules was 50g/667m2,the incidence of FCR was as low as 16.00%,the disease index was as low as 5.87,and the control effect was as high as 74.59%,indicating that its control effect was obviously better than the control effect of hymexazol(CK1).【Conclusion】Flutriafol had high antifungal activity against F.pseudograminearum,and had good control effect on FCR.1%flutriafol granules were mixed with compound fertilizer at a dosage of 50g/667m2,which had the best control effect on FCR.