真菌诱导白木香所结沉香质量分析

Quality Analysis of Agarwood Produced by Fungal in Duction of Aquilaria sinensis

为探究真菌诱导白木香Aquilaria sinensis结香7个月所产生沉香的主要化学成分及其质量是否符合行业标准,以石湾和星岛湖两地真菌诱导结香所产生的沉香为研究对象,测定其醇溶性提取物的含量,观察显色反应、采用高效液相分析其指纹图谱,利用气相色谱质谱联用仪(GC-MS)分析其主要化学成分。结果表明:石湾和星岛湖的沉香醇溶性提取物分别为(19.29±8.30)%、(22.54±5.96)%,显色反应呈现紫红色和淡紫色,高效液相特征图谱出现6个特征峰,符合沉香行业标准;石湾、星岛湖沉香分别具有12,15个倍半萜类物质,占30.80%、30.06%,分别具有4,5个2-(2-苯乙基)色酮类物质,占24.81%、30.62%。研究利用真菌结香剂诱导石湾和星岛湖两地的白木香结香,结香7个月的沉香可以达到《沉香》(LY/T 2904—2017)行业标准,并且倍半萜和2-(2-苯乙基)色酮类物质含量达到55.61%、60.08%。

To investigate whether the quality of agarwood produced after 7 months of fungal inducer Aquilaria sinensis meets industry standards and its main chemical components.The study focuses on agarwood induced by fungi in Shiwan and Xingdaohu for 7 months.The content of its alcohol-soluble extract was determined,its color reaction was observed,its fingerprint was analyzed using high-performance liquid chromatography,and its main chemical components were analyzed using gas chromatography-mass spectrometry(GCMS).The alcohol-soluble extracts of agarwood from Shiwan and Xingdaohu were(19.29±8.30)%and(22.54±5.96)%,respectively.The chromogenic reactions showed purplish red and light purple,and the high-performance liquid chromatography characteristic spectrum also showed 6 characteristic peaks,which met the agarwood industry standards;Shiwan and Xingdaohu agarwood had 12 and 15 Sesquiterpene,accounting for 30.80%and 30.06%respectively,4 and 52-(2-phenylethyl)chromones,accounting for 24.81%and 30.62%respectively.In this study,the agarwood of Shiwan and Xingdaohu for 7 months induced by fungal inducer can meet the industry standard of agarwood,and the content of Sesquiterpene and 2-(2-phenylethyl)chromones can reach 55.61%and 60.08%,providing new theory and method for artificial induction of agarwood.