摘要
该试验旨在探究不同染色剂对猪精子形态呈现效果的对比分析,试验选取3种染色剂(姬姆萨染色法、伊红苯胺黑染色法、精子蓝染色剂染色法)对长白猪精液开展精子染色。结果显示,精子蓝染色剂染色剂法猪精子头部和精子尾部染色效果最佳,精子头部轮廓和顶体清晰,精子形态呈现效果优于姬姆萨染色法和伊红苯胺黑染色法。综上可知,猪精子形态的呈现效果与染色液的染色效果有一定关系,精子蓝染色剂染色在猪精子形态的呈现上效果最佳,为检测人员更好的了解精子形态,更好的判断区分正常精子和畸形精子打下坚实基础,为质检人员在未来精子染色剂的选择上提供理论借鉴。
The experiment aims to explore the comparative analysis of the effects of different staining agents on the morphology of pig sperm.Three staining agents(Giemsa,eosin aniline black and spermblue)were selected to stain the sperm of Landrace pig semen.The results showed that the spermblue staining agent method had the best staining effect on the head and tail of pig sperm,with clear outline and acrosome of the sperm head,and better presentation of sperm morphology than the Giemsa staining method and the eosin aniline black staining method.In summary,there is a certain relationship between the presentation effect of pig sperm morphology and the staining effect of the staining solution.Spermblue staining has the best effect on the presentation of pig sperm morphology,laying a solid foundation for inspectors to better understand sperm morphology,distinguish normal sperm from abnormal sperm,and provide theoretical reference for quality inspectors in the selection of sperm staining agents in the future.
作者
李平
吴光松
姚敏
龙清孟
李俊
王元恒
申金容
曾金
张云淞
LI Ping;WU Guangsong;YAO Min;LONG Qingmeng;LI Jun;WANG Yuanheng;SHEN Jinrong;ZENG Jin;ZHANG Yunsong(The Livestock and Poultry Breeding Center in Guizhou Province,Guiyang Guizhou 550018,China;The Livestock and Poultry Variety Improvement Station in Kaiyang County,Kaiyang Guizhou 550330,China)
出处
《现代畜牧科技》
2024年第2期92-94,共3页
Modern Animal Husbandry Science & Technology
基金
贵州省科技支撑项目(黔科合支撑[2013]一般474)。
